Howe K, Williamson J, Boddy N, Sheer D, Freemont P, Solomon E
Division of Medical & Molecular Genetics, Guy's Dental School, Guy's Hospital, London, United Kingdom.
Genomics. 1998 Jan 1;47(1):92-100. doi: 10.1006/geno.1997.5091.
The human ubiquitin-homology domain protein PIC1 interacts with the acute promyelocytic leukemia protein PML, and both proteins form part of the large, nuclear, multiprotein complexes known as PML nuclear bodies. The normal punctate immunohistochemical staining pattern of these complexes is disrupted by viral infection or interferon treatment and in blast cells from patients with acute promyelocytic leukemia. We have characterized the murine homologue of PIC1 and have found that the predicted amino acid sequences of the mouse and human proteins are identical. High levels of Pic1 mRNA were detected in a range of mouse tissues. Pic1 genomic clones were isolated, and the organization of the gene was determined. Two processed Pic1 pseudogenes were also isolated and characterized. Through FISH, the chromosomal localizations of the mouse Pic1 gene and the two pseudogenes were determined. Human PIC1 (HGMW-approved symbol UBL1)-related sequences were isolated from human genomic DNA and were shown to represent processed pseudogenes. The role of PIC1 in a variety of cellular processes is discussed.
人类泛素同源结构域蛋白PIC1与急性早幼粒细胞白血病蛋白PML相互作用，这两种蛋白都是被称为PML核体的大型核多蛋白复合物的组成部分。这些复合物正常的点状免疫组化染色模式在病毒感染、干扰素治疗以及急性早幼粒细胞白血病患者的原始细胞中会被破坏。我们已经鉴定了PIC1的小鼠同源物，发现小鼠和人类蛋白质的预测氨基酸序列是相同的。在一系列小鼠组织中检测到高水平的Pic1 mRNA。分离出了Pic1基因组克隆，并确定了该基因的结构。还分离并鉴定了两个加工后的Pic1假基因。通过荧光原位杂交确定了小鼠Pic1基因和两个假基因的染色体定位。从人类基因组DNA中分离出了与人类PIC1（HGMW认可符号UBL1）相关的序列，并显示它们代表加工后的假基因。文中讨论了PIC1在多种细胞过程中的作用。
