用于检测人类免疫缺陷病毒1型pol基因中与齐多夫定、去羟肌苷和拉米夫定耐药性相关突变的寡核苷酸连接测定法。
Oligonucleotide ligation assay for detecting mutations in the human immunodeficiency virus type 1 pol gene that are associated with resistance to zidovudine, didanosine, and lamivudine.
作者信息
Edelstein R E, Nickerson D A, Tobe V O, Manns-Arcuino L A, Frenkel L M
机构信息
Department of Pediatrics, University of Washington, Seattle, USA.
出版信息
J Clin Microbiol. 1998 Feb;36(2):569-72. doi: 10.1128/JCM.36.2.569-572.1998.
Abstract
This report describes the detection of mutations in the pol gene of human immunodeficiency virus type 1 associated with resistance to zidovudine, didanosine, and lamivudine by genotyping by an oligonucleotide ligation assay specific codons in the pol gene amplified by PCR. Our studies demonstrate the sensitivity, simplicity, and specificity of this genotyping system.
摘要
本报告描述了通过聚合酶链反应（PCR）扩增pol基因中的特定密码子，采用寡核苷酸连接测定法进行基因分型，检测1型人类免疫缺陷病毒pol基因中与齐多夫定、去羟肌苷和拉米夫定耐药相关的突变。我们的研究证明了该基因分型系统的敏感性、简易性和特异性。
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Oligonucleotide ligation assay for detecting mutations in the human immunodeficiency virus type 1 pol gene that are associated with resistance to zidovudine, didanosine, and lamivudine.用于检测人类免疫缺陷病毒1型pol基因中与齐多夫定、去羟肌苷和拉米夫定耐药性相关突变的寡核苷酸连接测定法。
J Clin Microbiol. 1998 Feb;36(2):569-72. doi: 10.1128/JCM.36.2.569-572.1998.
2
Specific, sensitive, and rapid assay for human immunodeficiency virus type 1 pol mutations associated with resistance to zidovudine and didanosine.针对与齐多夫定和去羟肌苷耐药相关的人类免疫缺陷病毒1型 pol 突变的特异性、灵敏且快速的检测方法。
J Clin Microbiol. 1995 Feb;33(2):342-7. doi: 10.1128/jcm.33.2.342-347.1995.
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J Clin Virol. 2000 Dec;19(3):135-42. doi: 10.1016/s1386-6532(00)00087-1.
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Specific, sensitive, and rapid assay for human immunodeficiency virus type 1 pol mutations associated with resistance to zidovudine and didanosine.针对与齐多夫定和去羟肌苷耐药相关的人类免疫缺陷病毒1型 pol 突变的特异性、灵敏且快速的检测方法。
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