Winzeler E A, Davis R W
Department of Biochemistry, Stanford University School of Medicine, California 94305-5307, USA.
Curr Opin Genet Dev. 1997 Dec;7(6):771-6. doi: 10.1016/s0959-437x(97)80039-1.
The release of the complete genome sequence of the yeast Saccharomyces cerevisiae has ushered in a new phase of genome research in which sequence function will be assigned. The goal is to determine the biological function of each of the > 6,000 open reading frames in the yeast genome. Innovative approaches have been developed that exploit the sequence data and yield information about gene expression levels, protein levels, subcellular localization and gene function for the entire genome.
酿酒酵母完整基因组序列的公布开启了一个新的基因组研究阶段，在此阶段将对序列功能进行注释。目标是确定酵母基因组中6000多个开放阅读框各自的生物学功能。已经开发出了创新方法，这些方法利用序列数据，可得出关于整个基因组的基因表达水平、蛋白质水平、亚细胞定位及基因功能的信息。
