Landa A S, van der Mei H C, Busscher H J
Laboratory for Materia Technica, University of Groningen, The Netherlands.
Adv Dent Res. 1997 Nov;11(4):528-38. doi: 10.1177/08959374970110042201.
The biofilm mode of growth protects plaque micro-organisms against environmental attacks, such as from antimicrobials or detergents. Dental plaque is linked to enamel through the adhesion of initial colonizers. Once this link is disrupted, the entire plaque mass adhering to it detaches. Experiments in a parallel-plate flow chamber demonstrated that bacteria adhering to saliva-coated enamel could not be stimulated to detach by perfusion of the flow chamber with two traditional mouthrinses (Corsodyl and Scope), whereas perfusion with a prebrushing rinse (Plax) or its detergent components stimulated detachment from saliva-coated enamel of a wide variety of bacterial strains. Following perfusion of the flow chamber with the mouthrinses, little additional detachment of adhering bacteria by the passage of a liquid-air interface occurred. After perfusion with the prebrushing rinse, however, significant numbers of still-adhering bacteria could be stimulated to detach by passage of a liquid-air interface, indicating that Plax had weakened their adhesive bond. The ability of Plax or its detergent components to detach plaque bacteria is not always obvious from in vivo experiments, and reports on its clinical efficacy are inconsistent. Likely, antimicrobials or detergents are unable to penetrate the plaque and reach the linking film bacteria, as demonstrated here by Fourier transform infrared spectroscopy.
生物膜生长模式可保护牙菌斑微生物免受环境攻击，如抗菌剂或洗涤剂的攻击。牙菌斑通过早期定植菌的黏附与牙釉质相连。一旦这种连接被破坏，附着在其上的整个菌斑团块就会脱落。在平行平板流动腔中的实验表明，用两种传统漱口水（高露洁全效和Scope）灌注流动腔，无法刺激附着在唾液包被牙釉质上的细菌脱落，而用刷牙前预冲洗液（Plax）或其洗涤剂成分灌注，则可刺激多种细菌菌株从唾液包被的牙釉质上脱落。用漱口水灌注流动腔后，通过液 - 气界面的通过几乎不会使附着的细菌进一步脱落。然而，用刷牙前预冲洗液灌注后，通过液 - 气界面的通过可刺激大量仍附着的细菌脱落，这表明Plax削弱了它们的黏附力。从体内实验中，Plax或其洗涤剂成分使菌斑细菌脱落的能力并不总是明显的，关于其临床疗效的报道也不一致。抗菌剂或洗涤剂可能无法穿透菌斑并到达连接膜细菌，傅里叶变换红外光谱在此处的研究证明了这一点。
