Yajid F, Mercier J G, Mercier B M, Dubouchaud H, Préfaut C
Laboratoire de Physiologie des Interactions, Hôpital Arnaud de Villeneuve, Montpellier, France.
J Appl Physiol (1985). 1998 Feb;84(2):479-85. doi: 10.1152/jappl.1998.84.2.479.
We investigated in rats the effect of 4 wk of hypodynamia on the respiration of mitochondria isolated from four distinct muscles [soleus, extensor digitorum longus, tibial anterior, and gastrocnemius (Gas)] and from subsarcolemmal (SS) and intermyofibrillar (IMF) regions of mixed hindlimb muscles that mainly contained the four cited muscles. With pyruvate plus malate as respiratory substrate, 4 wk of hindlimb suspension produced an 18% decrease in state 3 respiration for IMF mitochondria compared with those in the control group (P < 0.05). The SS mitochondria state 3 were not significantly changed. Concerning the four single muscles, the mitochondrial respiration was significantly decreased in the Gas muscle, which showed a 59% decrease in state 3 with pyruvate + malate (P < 0.05). The other muscles presented no significant decrease in respiratory rate in comparison with the control group. With succinate + rotenone, there was no significant difference in the respiratory rate compared with the respective control group, whatever the mitochondrial origin (SS, or IMF, or from single muscle). We conclude that 4 wk of hindlimb suspension alters the respiration of IMF mitochondria in hindlimb skeletal muscles and seems to act negatively on complex I of the electron-transport chain or prior sites. The muscle mitochondria most affected are those isolated from the Gas muscle.
我们在大鼠中研究了4周低动力状态对从四种不同肌肉[比目鱼肌、趾长伸肌、胫骨前肌和腓肠肌（Gas）]以及主要包含上述四种肌肉的混合后肢肌肉的肌膜下（SS）和肌原纤维间（IMF）区域分离出的线粒体呼吸的影响。以丙酮酸加苹果酸作为呼吸底物时，与对照组相比，4周后肢悬吊使IMF线粒体的状态3呼吸下降了18%（P<0.05）。SS线粒体的状态3没有显著变化。对于四块单一肌肉，Gas肌肉的线粒体呼吸显著下降，丙酮酸+苹果酸时状态3下降了59%（P<0.05）。与对照组相比，其他肌肉的呼吸速率没有显著下降。使用琥珀酸+鱼藤酮时，无论线粒体来源（SS、IMF或单一肌肉）如何，与各自对照组相比，呼吸速率均无显著差异。我们得出结论，4周后肢悬吊会改变后肢骨骼肌中IMF线粒体的呼吸，并且似乎对电子传递链的复合体I或其之前的位点产生负面影响。受影响最大的肌肉线粒体是从Gas肌肉分离出的那些。
