Menghi G, Bondi A M, Marchetti L, Gabrielli M G, Materazzi G
Department of Cellular Molecular and Animal Biology, University of Camerino, MC, Italy.
Histol Histopathol. 1998 Jan;13(1):137-46. doi: 10.14670/HH-13.137.
An ultrastructural analysis of lectin receptors on the submandibular glands from mice of both sexes was performed utilizing horseradish peroxidase-labelled lectins in conjunction with antiperoxidase antibody and protein A-gold. Both qualitative and quantitative sex-related differences in terminal sugar expression within secretory granules were detected. Following sialidase digestion, also subterminal acceptor sugars for terminal sialic acids, proved to be differentially expressed in the submandibular glands of males and females. Heterogeneous distribution of sialoglycoconjugates characterized by the terminal disaccharide sialic acid-beta-galactose was found to occur in female acinar cells. Also DBA reactive sites indicating the presence of terminal alpha-N-acetylgalactosamine discriminated between male and female acinar secretory glycoconjugates. This difference was emphasized by sialidase pretreatment that evidenced a marked occurrence of sialic acid subtended to alpha-N-acetylgalactosamine in males in contrast to a modest presence in females. The different sialylation patterns of acinar cell secretory products, probably related to a different expression of O- and N-linked sialoglycoconjugates, give insight into the sexual dimorphism of the mouse submandibular gland known until recently for the convoluted granular tubules.
利用辣根过氧化物酶标记的凝集素结合抗过氧化物酶抗体和蛋白A-金，对两性小鼠下颌下腺的凝集素受体进行了超微结构分析。在分泌颗粒内的末端糖表达方面检测到了与性别相关的定性和定量差异。唾液酸酶消化后，末端唾液酸的亚末端受体糖在雄性和雌性小鼠的下颌下腺中也被证明存在差异表达。在雌性腺泡细胞中发现了以末端二糖唾液酸-β-半乳糖为特征的唾液糖缀合物的异质分布。同样，表明存在末端α-N-乙酰半乳糖胺的DBA反应位点也区分了雄性和雌性腺泡分泌糖缀合物。唾液酸酶预处理强调了这种差异，该预处理表明，与雌性中适度存在相比，雄性中α-N-乙酰半乳糖胺所携带的唾液酸显著存在。腺泡细胞分泌产物的不同唾液酸化模式，可能与O-和N-连接唾液糖缀合物的不同表达有关，这为直到最近才因迂曲颗粒小管而闻名的小鼠下颌下腺的性别二态性提供了见解。
