Giacomelli R, Matucci-Cerinic M, Cipriani P, Ghersetich I, Lattanzio R, Pavan A, Pignone A, Cagnoni M L, Lotti T, Tonietti G
University of L'Aquila, Italy.
Arthritis Rheum. 1998 Feb;41(2):327-34. doi: 10.1002/1529-0131(199802)41:2<327::AID-ART17>3.0.CO;2-S.
An increased percentage of Vdelta1+/gamma/delta T cells has been detected both in the peripheral blood and bronchoalveolar lavage fluid of patients with systemic sclerosis (SSc). This study evaluated the subset distribution, activation status, and expression of cellular adhesion molecules, such as intercellular adhesion molecule 1 (CD54), very late activation antigen alpha4 (CD49d), and lymphocyte function-associated antigen 1alpha (CD11a), on circulating gamma/delta T cells, as well as their presence in the skin of SSc patients.
We studied 12 patients with SSc and 16 healthy volunteer donors. The distribution, activation status, and expression of cellular adhesion molecules were studied by flow cytometry; their presence in SSc patient skin was evaluated by immunohistochemistry.
We found that the percentages and absolute numbers of peripheral blood gamma/delta T cells, CD16, CD8, CD45RO, CD25, HLA-DR, CD54, and CD11a coexpression did not differ significantly from those of the controls. CD49d gamma/delta T cells were significantly increased in SSc patients (2.3%) compared with controls (0.5%). A marked increase in the ratio of Vdelta1+ cells to gamma/delta cells was observed in the patients (72%) compared with the controls (31%). The Vdelta1+ subset showed a significant expression of both HLA-DR (83% of total Vdelta1+ cells) and CD49d (90% of total Vdelta1+ cells) compared with the controls (20.5% and 60%, respectively). In the skin, the absolute numbers of gamma/delta T cells were found in striking amounts in perivascular areas, particularly in the early edematous phase of SSc (22.58 in patients and 0 in controls); the majority of gamma/delta T cells were Vdelta1+ (19 in patients and 0 in controls). In the advanced phase of SSc, Vdelta1+ T cells were also increased compared with controls (3.5 versus 0).
Our results show that Vdelta1+ T cells express both adhesion molecules and activation markers, and strongly support gamma/delta T cell homing to sites of inflammation. The increase in the Vdelta1 subset suggests a selective V gene subset expansion.
在系统性硬化症（SSc）患者的外周血和支气管肺泡灌洗液中均检测到Vδ1⁺/γ/δ T细胞百分比增加。本研究评估了循环γ/δ T细胞上细胞间粘附分子1（CD54）、极晚期活化抗原α4（CD49d）和淋巴细胞功能相关抗原1α（CD11a）等细胞粘附分子的亚群分布、活化状态及表达情况，以及它们在SSc患者皮肤中的存在情况。
我们研究了12例SSc患者和16名健康志愿者供体。通过流式细胞术研究细胞粘附分子的分布、活化状态及表达；通过免疫组织化学评估它们在SSc患者皮肤中的存在情况。
我们发现外周血γ/δ T细胞、CD16、CD8、CD45RO、CD25、HLA-DR、CD54和CD11a共表达的百分比及绝对数量与对照组相比无显著差异。与对照组（0.5%）相比，SSc患者中CD49d γ/δ T细胞显著增加（2.3%）。与对照组（31%）相比，患者中Vδ1⁺细胞与γ/δ细胞的比例显著增加（72%）。与对照组（分别为20.5%和60%）相比，Vδ1⁺亚群显示HLA-DR（占总Vδ1⁺细胞的83%）和CD49d（占总Vδ1⁺细胞的90%）均有显著表达。在皮肤中，γ/δ T细胞的绝对数量在血管周围区域大量存在，特别是在SSc的早期水肿阶段（患者为22.58，对照组为0）；大多数γ/δ T细胞为Vδ1⁺（患者为19，对照组为0）。在SSc的晚期，与对照组相比Vδ1⁺ T细胞也增加（3.5对0）。
我们的结果表明Vδ1⁺ T细胞表达粘附分子和活化标志物，并有力支持γ/δ T细胞归巢至炎症部位。Vδ1亚群的增加提示V基因亚群选择性扩增。
