使用生物指示剂对新灭菌技术的评估。
Evaluation of new technique of sterilization using biological indicator.
作者信息
Sheth Nomal Chintan, Rathod Yogesh V, Shenoi Pratima R, Shori Deepa D, Khode Rajiv T, Khadse Amruta P
机构信息
Department of Conservative Dentisty and Endodontics, VSPM Dental College and Research Center, Nagpur, Maharashtra, India.
出版信息
J Conserv Dent. 2017 Sep-Oct;20(5):346-350. doi: 10.4103/JCD.JCD_253_16.
BACKGROUND
A novel technique of sterilization of endodontic files is introduced in this article.
AIMS
Newly introduced sterilization unit, named "SteriFast" is compared with autoclave and glass bead sterilizer using biological indicator.
MATERIALS AND METHODS
Spore strips of were cultured in nutrient broth. This cultured media was used to contaminate the experimental samples of endodontic files. These contaminated files were sterilized using three different techniques. The sterilized files were transferred into nutrient medium under aseptic condition. The results were observed after 24 h, 48 h, and 7 days.
RESULTS
The results showed that autoclave and new sterilization device (SteriFast) showed complete sterilization. The files sterilized using glass bead sterilizer showed bacterial growth (80%).
CONCLUSIONS
Thus, it proves that autoclave and SteriFast are ideal techniques of sterilization of endodontic files. Glass bead sterilizer does not completely sterilize the files. The article also compares SteriFast and autoclave in other aspects such as its design, basic principle, advantages, and disadvantages. The article also describes features and design of SteriFast, used for all kind of small dental instruments.
背景
本文介绍了一种新型的根管锉消毒技术。
目的
使用生物指示剂，将新推出的名为“SteriFast”的消毒设备与高压灭菌器和玻璃珠消毒器进行比较。
材料与方法
将芽孢条在营养肉汤中培养。这种培养基用于污染根管锉的实验样本。这些受污染的锉用三种不同技术进行消毒。消毒后的锉在无菌条件下转移到营养培养基中。在24小时、48小时和7天后观察结果。
结果
结果显示高压灭菌器和新消毒设备（SteriFast）显示完全消毒。使用玻璃珠消毒器消毒的锉显示有细菌生长（80%）。
结论
因此，证明高压灭菌器和SteriFast是根管锉消毒的理想技术。玻璃珠消毒器不能完全对锉进行消毒。本文还在设计、基本原理、优点和缺点等其他方面比较了SteriFast和高压灭菌器。本文还描述了用于所有小型牙科器械的SteriFast的特点和设计。
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