空肠弯曲菌NCTC 11168（UA580）改进的物理图谱和遗传图谱。
An improved physical and genetic map of Campylobacter jejuni NCTC 11168 (UA580).
作者信息
Karlyshev Andrey V, Henderson John, Ketley Julian M, Wren Brendan W
机构信息
Department of Medical Microbiology, St Bartholomew's and the Royal London School of Medicine and Dentistry, West Smithfield, London EC1A 7BE, UK.
Department of Genetics, University of Leicester, Leicester LE1 7RH, UK.
出版信息
Microbiology (Reading). 1998 Feb;144 ( Pt 2):503-508. doi: 10.1099/00221287-144-2-503.
Campylobacter jejuni is recognized as the major cause of food-borne gastrointestinal disease in the developed world. To facilitate the molecular genetic analysis of this pathogen, an approximately 18-fold redundant Tropist3 cosmid library was constructed from C. jejuni NCTC 11168 genomic DNA. The cosmid library was partially ordered by hybridization to 15 pulsed-field electrophoresis (PFGE) restriction fragments. This analysis confirmed the estimated size of the genome to be 1730 kb, but suggested discrepancies in some regions of the published physical map. The precise locations of two of the three rRNA gene clusters were mapped using a combination of restriction fingerprinting, sample sequencing and riboprobing. Additionally, 15 further genes were located on the revised map. A more detailed physical and genetic map of C. jejuni NCTC 11168 is presented.
空肠弯曲菌被认为是发达国家食源性胃肠疾病的主要病因。为便于对该病原体进行分子遗传学分析，用空肠弯曲菌NCTC 11168基因组DNA构建了一个约18倍冗余的Tropist3黏粒文库。通过与15个脉冲场凝胶电泳（PFGE）限制性片段杂交对黏粒文库进行了部分排序。该分析证实基因组估计大小为1730 kb，但表明已发表的物理图谱某些区域存在差异。使用限制性指纹图谱、样本测序和核糖核酸探针相结合的方法对三个rRNA基因簇中的两个的精确位置进行了定位。此外，在修订后的图谱上定位了另外15个基因。本文给出了空肠弯曲菌NCTC 11168更详细的物理和遗传图谱。
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