胞质Gag p24作为1型人类免疫缺陷病毒有效进入的指标。
Cytosolic Gag p24 as an index of productive entry of human immunodeficiency virus type 1.
作者信息
Maréchal V, Clavel F, Heard J M, Schwartz O
机构信息
Laboratoire Rétrovirus et Transfert Génétique, CNRS URA 1157, Institut Pasteur, Paris, France.
出版信息
J Virol. 1998 Mar;72(3):2208-12. doi: 10.1128/JVI.72.3.2208-2212.1998.
Abstract
We have investigated the cellular uptake of Gag p24 shortly after exposure of cells to human immunodeficiency virus (HIV) particles. In the absence of envelope glycoprotein on virions or of viral receptors or coreceptors at the cell surface, p24 was incorporated in intracellular vesicles but not detected in the cytosolic subcellular fraction. When appropriate envelope-receptor interactions could occur, the nonspecific vesicular uptake was still intense and cytosolic p24 represented 10 to 40% of total intracellular p24. The measurement of cytosolic p24 early after exposure to HIV type 1 is a reliable assay for investigating virus entry and early events leading to authentic cell infection.
摘要
我们研究了细胞暴露于人类免疫缺陷病毒（HIV）颗粒后不久Gag p24的细胞摄取情况。在病毒粒子上不存在包膜糖蛋白或细胞表面不存在病毒受体或共受体的情况下，p24被纳入细胞内囊泡，但在胞质亚细胞部分未检测到。当适当的包膜-受体相互作用能够发生时，非特异性囊泡摄取仍然强烈，胞质p24占细胞内总p24的10%至40%。暴露于1型HIV后早期对胞质p24的测量是一种用于研究病毒进入以及导致真正细胞感染的早期事件的可靠检测方法。
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