软骨细胞衍生的凋亡小体与关节软骨钙化
Chondrocyte-derived apoptotic bodies and calcification of articular cartilage.
作者信息
Hashimoto S, Ochs R L, Rosen F, Quach J, McCabe G, Solan J, Seegmiller J E, Terkeltaub R, Lotz M
机构信息
Division of Arthritis Research, The Scripps Research Institute, La Jolla, CA 92037, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3094-9. doi: 10.1073/pnas.95.6.3094.
Abstract
Chondrocytes exposed to nitric oxide (NO) or antibody to Fas undergo cell death by apoptosis. This study examines structural and functional properties of chondrocyte-derived apoptotic bodies. In NO treated cartilage, the dense pericellular matrix that normally surrounds the cells is degraded and apoptotic bodies accumulate within and in the vicinity of the chondrocyte lacunae. Functional analysis shows that apoptotic bodies isolated from NO-treated chondrocytes or cartilage produce pyrophosphate. The levels of pyrophosphate produced by apoptotic bodies are increased by pretreatment of the chondrocytes with transforming growth factor beta and decreased by interleukin 1. Apoptotic bodies contain alkaline phosphatase and NTP pyrophosphohydrolase activities and can precipitate calcium. These results suggest that chondrocyte-derived apoptotic bodies express functional properties that may contribute to the pathologic cartilage calcification observed in aging and osteoarthritis.
摘要
暴露于一氧化氮（NO）或Fas抗体的软骨细胞会通过凋亡发生细胞死亡。本研究检测了软骨细胞衍生的凋亡小体的结构和功能特性。在经NO处理的软骨中，正常情况下围绕细胞的致密细胞周基质会降解，凋亡小体在软骨细胞腔隙内及附近积聚。功能分析表明，从经NO处理的软骨细胞或软骨中分离出的凋亡小体可产生焦磷酸盐。用转化生长因子β预处理软骨细胞可增加凋亡小体产生的焦磷酸盐水平，而白细胞介素1则可降低其水平。凋亡小体含有碱性磷酸酶和NTP焦磷酸水解酶活性，并且能够沉淀钙。这些结果表明，软骨细胞衍生的凋亡小体表达的功能特性可能导致在衰老和骨关节炎中观察到的病理性软骨钙化。
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