Cassey B, Guest J R, Attwood M M
Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
FEMS Microbiol Lett. 1998 Feb 15;159(2):325-9. doi: 10.1111/j.1574-6968.1998.tb12878.x.
The effects of changing environmental conditions on expression of the pdh operon were studied in strains containing pyruvate dehydrogenase (PDH) complexes having either one or three lipoyl domains per lipoate acetyltransferase chain. The expression of the pdh operon was lowered during growth on reduced carbon sources and when the mode of energy generation was changed from aerobic respiration to anaerobic respiration and fermentation. In contrast, growth at non-optimal pH increased expression. Operon expression was generally higher in the 1 lip strain compared to the 3 lip strain. Expression of the pdh operon was shown to be tightly controlled in response to environmental stimuli, consistent with its importance in defining metabolic flux.
在含有丙酮酸脱氢酶（PDH）复合物的菌株中，研究了环境条件变化对pdh操纵子表达的影响，这些复合物的每个硫辛酸乙酰转移酶链含有一个或三个硫辛酰结构域。在以还原碳源为底物生长时，以及当能量产生模式从有氧呼吸转变为无氧呼吸和发酵时，pdh操纵子的表达会降低。相比之下，在非最适pH值下生长会增加表达。与3 lip菌株相比，1 lip菌株中的操纵子表达通常更高。结果表明，pdh操纵子的表达受到环境刺激的严格控制，这与其在定义代谢通量中的重要性相一致。
