Appel R D, Vargas J R, Palagi P M, Walther D, Hochstrasser D F
Medical Informatics Division, Geneva University Hospital, Switzerland.
Electrophoresis. 1997 Dec;18(15):2735-48. doi: 10.1002/elps.1150181507.
After two generations of software systems for the analysis of two-dimensional electrophoresis (2-DE) images, a third generation of such software packages has recently emerged that combines state-of-the-art graphical user interfaces with comprehensive spot data analysis capabilities. A key characteristic common to most of these software packages is that many of their tools are implementations of algorithms that resulted from research areas such as image processing, vision, artificial intelligence or machine learning. This article presents the main algorithms implemented in the Melanie II 2-D PAGE software package. The applications of these algorithms, embodied as the feature of the program, are explained in an accompanying article (R. D. Appel et al.; Electrophoresis 1997, 18, 2724-2734).
在经历了两代用于二维电泳（2-DE）图像分析的软件系统之后，最近出现了第三代此类软件包，它将最先进的图形用户界面与全面的斑点数据分析能力相结合。这些软件包中的大多数都具有一个关键特性，即它们的许多工具都是源于图像处理、视觉、人工智能或机器学习等研究领域的算法实现。本文介绍了Melanie II二维聚丙烯酰胺凝胶电泳软件包中实现的主要算法。这些算法的应用作为该程序的功能，在随附的一篇文章中进行了说明（R. D. Appel等人；《电泳》，1997年，18卷，2724 - 2734页）。
