腺病毒转化细胞的mRNA与腺病毒基因组片段的杂交。
Hybridization of mRNA from adenovirus-transformed cells to segments of the adenovirus genome.
作者信息
Bachenheimer S, Darnell J E
出版信息
J Virol. 1976 Jul;19(1):286-9. doi: 10.1128/JVI.19.1.286-289.1976.
Abstract
Cytoplasmic viral-specific RNA from the 8617 line of adenovirus type 2 (Ad2) transformed rat cells was hybridized to DNA fragments derived either by Eco-RI or Sma-I restriction endonucleases. Three discrete classes of viral RNA (26S, 20S, and 15S) were detected by hybridization of total cytoplasmic RNA with the fragment, and all of these RNA molecules also hybridized to the much smaller Sma-E fragment of Ad2 DNA. Poly (A)-containing RNA (exposed to 90% formamide) contained only the 20S and 15S mRNA species, suggesting that the 26S RNA might be an aggregated species. Viral RNA of the same size derived from the same regions of the genome has not been detected in lytically infected cells, suggesting some change in the pattern of transcription or RNA processing of the Ad2 DNA in the integrated state.
摘要
来自腺病毒2型（Ad2）转化大鼠细胞8617系的细胞质病毒特异性RNA与由Eco - RI或Sma - I限制性内切酶产生的DNA片段进行杂交。通过总细胞质RNA与该片段杂交检测到三类离散的病毒RNA（26S、20S和15S），并且所有这些RNA分子也与Ad2 DNA小得多的Sma - E片段杂交。含聚腺苷酸的RNA（暴露于90%甲酰胺）仅包含20S和15S mRNA种类，这表明26S RNA可能是一种聚集物。在裂解感染的细胞中未检测到来自基因组相同区域的相同大小的病毒RNA，这表明整合状态下Ad2 DNA的转录或RNA加工模式存在一些变化。
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