用于扩增2型人类免疫缺陷病毒DNA的高度灵敏方法。
Highly sensitive method for amplification of human immunodeficiency virus type 2 DNA.
作者信息
Damond F, Loussert-Ajaka I, Apetrei C, Descamps D, Souquière S, Leprêtre A, Matheron S, Brun-Vézinet F, Simon F
机构信息
Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, Paris, France.
出版信息
J Clin Microbiol. 1998 Mar;36(3):809-11. doi: 10.1128/JCM.36.3.809-811.1998.
Abstract
We evaluated a new human immunodeficiency virus type 2 (HIV-2) DNA amplification strategy based on peripheral blood mononuclear cell long PCR (XL PCR) followed by nested PCR amplification. The primers used were located in the highly conserved long terminal repeat and in the pol regions of the genome. Five primer pairs corresponding to different regions of the HIV-2 env gene were used in the nested step. Samples from 42 patients were tested, which yielded positive amplification with at least two primer pairs in 40 (95%) samples. A primer pair (EB2/EB5) located on the V3 region succeeded in amplifying proviral DNA in 40 samples.
摘要
我们评估了一种基于外周血单核细胞长程聚合酶链反应（XL PCR）随后进行巢式PCR扩增的新型2型人类免疫缺陷病毒（HIV-2）DNA扩增策略。所使用的引物位于基因组高度保守的长末端重复序列和pol区域。在巢式扩增步骤中使用了对应HIV-2 env基因不同区域的五对引物。对42例患者的样本进行了检测，其中40例（95%）样本通过至少两对引物产生了阳性扩增。位于V3区域的一对引物（EB2/EB5）成功地在40个样本中扩增出了前病毒DNA。
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