上半部分的tDNA小螺旋是真细菌CCA添加酶的良好底物。
A top-half tDNA minihelix is a good substrate for the eubacterial CCA-adding enzyme.
作者信息
Shi P Y, Weiner A M, Maizels N
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520-8024, USA.
出版信息
RNA. 1998 Mar;4(3):276-84.
The CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase] catalyzes the addition and regeneration of the 3'-terminal CCA sequence of tRNAs. We show that the CCA-adding enzyme will specifically add a CCA terminus to synthetic full-length tDNA and to DNA oligonucleotides corresponding to the "top half" of tRNA-the acceptor stem and TpsiC stem-loop of tRNA. CCA addition to the top half tDNA minihelices requires a 2' as well as a 3' OH at the 3' terminus of the tDNA. Addition also depends on the length of the base paired stem, and is facilitated by, but is not dependent upon, the presence of a TpsiC loop. These results provide further evidence for independent functions of the top and bottom halves of tRNA, and support the hypothesis that these two structurally distinct and functionally independent domains evolved independently.
CCA添加酶[ATP(CTP)：tRNA核苷酸转移酶]催化tRNA 3'末端CCA序列的添加和再生。我们发现，CCA添加酶能特异性地将CCA末端添加到合成的全长tDNA以及与tRNA“上半部分”相对应的DNA寡核苷酸上，tRNA的“上半部分”即tRNA的受体茎和TψC茎环。将CCA添加到上半部分tDNA小螺旋需要tDNA 3'末端同时具有2'和3'羟基。添加还取决于碱基配对茎的长度，并且TψC环的存在有助于添加，但并非必需。这些结果为tRNA上下两半部分的独立功能提供了进一步的证据，并支持了这两个结构不同且功能独立的结构域独立进化的假说。
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