Inoue Y, Sugiyama K, Izawa S, Kimura A
Research Institute for Food Science, Kyoto University, Japan.
Biochim Biophys Acta. 1998 Feb 11;1395(3):315-20. doi: 10.1016/s0167-4781(97)00199-1.
The hypothetical protein YOL049w on the chromosome XV was identified to be the structural gene for glutathione synthetase (GSH2) of Saccharomyces cerevisiae. Translational initiation site was identified by making the GSH2-lacZ fusion. The GSH2 gene contained an open reading frame (1473 bp) with 491 amino acids, and molecular weight of the GSH2 gene product was calculated to be 55,812. Glutathione synthetase activity in transformant carrying the GSH2 gene with multicopy plasmid increased approximately 4-fold. The GSH2 gene was not essential for growth of yeast cell, and glutathione was not detected from the gsh2 disrupter.
已确定位于第十五号染色体上的假定蛋白YOL049w是酿酒酵母谷胱甘肽合成酶（GSH2）的结构基因。通过构建GSH2-lacZ融合体确定了翻译起始位点。GSH2基因包含一个1473 bp的开放阅读框，编码491个氨基酸，计算得出GSH2基因产物的分子量为55812。携带多拷贝质粒的GSH2基因的转化体中的谷胱甘肽合成酶活性增加了约4倍。GSH2基因对于酵母细胞的生长并非必需，并且在gsh2破坏株中未检测到谷胱甘肽。
