Paul M L, Pegler M A, Benn R A
Department of Microbiology, Royal Prince Alfred Hospital, Camperdown, NSW, Australia.
J Hosp Infect. 1998 Jan;38(1):19-26. doi: 10.1016/s0195-6701(98)90171-2.
Forty individual patient sputum isolates of Burkholderia cepacia from two Australian cystic fibrosis (CF) centres more than 100 km apart were genotyped using pulsed-field gel electrophoresis (PFGE) with XbaI restriction enzyme digestion. Hospital 1 had an endemic strain with 19 of 20 isolates being closely related. This centre does not implement an inpatient segregation policy for its paediatric patients who constitute the majority of those colonized with B. cepacia. Hospital 2 did not have a single endemic strain; there were two different sibling clusters and a third cluster involving a cohabiting couple, but all other patients had unique isolates. One patient at Hospital 2 carried an organism closely related to the endemic strain from Hospital 1. Hospital 2 practises segregation of colonized inpatients and also segregation external to the hospital. It would appear that no nosocomial spread of infection is occurring with this policy.
利用XbaI限制性内切酶消化的脉冲场凝胶电泳（PFGE）技术，对来自澳大利亚两个相距100多公里的囊性纤维化（CF）中心的40株洋葱伯克霍尔德菌患者痰液分离株进行了基因分型。医院1有一种地方流行菌株，20株分离株中有19株密切相关。该中心没有对其儿科患者实施住院隔离政策，这些儿科患者是大多数被洋葱伯克霍尔德菌定植的患者。医院2没有单一的地方流行菌株；有两个不同的同胞簇和第三个涉及一对同居夫妇的簇，但所有其他患者都有独特的分离株。医院2的一名患者携带的一种微生物与医院1的地方流行菌株密切相关。医院2对定植的住院患者进行隔离，并且在医院外部也进行隔离。看来这项政策没有导致医院感染的传播。
