Marcus D L, Thomas C, Rodriguez C, Simberkoff K, Tsai J S, Strafaci J A, Freedman M L
Division of Geriatrics, NYU Medical Center, New York, New York 10016, USA.
Exp Neurol. 1998 Mar;150(1):40-4. doi: 10.1006/exnr.1997.6750.
The overall peroxidation activity in brain tissue by region from patients with Alzheimer's disease (AD) and age-matched controls was determined employing the thiobarbituric acid-reactive substances (TBARS) assay, a measure of lipid peroxidation, followed by a determination the activities of the antioxidant enzymes Cu/Zn superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT), in the frontal, temporal, and cerebellar cortex of 10 AD and 9 control brains. The level of TBARS was elevated in all regions, with particular statistical significance in the temporal cortex when compared to age-matched controls. SOD activity was significantly decreased in AD frontal and AD temporal cortex, while catalase activity was significantly decreased in AD temporal cortex. There was no significant difference in GSH-Px activity found in any of the regions examined. This study supports the theory that in AD the brain is affected by increased oxidative stress which, when combined with a decrease in SOD activity, produces oxidative alterations, seen most significantly in temporal cortex in AD, where the pathophysiologic changes are most severe.
采用硫代巴比妥酸反应性物质（TBARS）测定法（一种脂质过氧化的测量方法），对10例阿尔茨海默病（AD）患者和9例年龄匹配的对照者大脑各区域的总体过氧化活性进行了测定，随后测定了10例AD患者和9例对照者大脑额叶、颞叶和小脑皮质中抗氧化酶铜/锌超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）的活性。所有区域的TBARS水平均升高，与年龄匹配的对照者相比，颞叶皮质具有特别的统计学意义。AD患者额叶和颞叶皮质的SOD活性显著降低，而AD患者颞叶皮质的过氧化氢酶活性显著降低。在所检查的任何区域中，GSH-Px活性均未发现显著差异。这项研究支持了这样一种理论，即在AD中，大脑受到氧化应激增加的影响，当与SOD活性降低相结合时，会产生氧化改变，在AD的颞叶皮质中最为明显，那里的病理生理变化最为严重。
