红细胞悬液介质对血凝反应和抗球蛋白试验的影响。

The effects of red blood cell suspending media on hemagglutination and the antiglobulin test.

作者信息

Fitzsimmons J M, Morel P A

出版信息

Transfusion. 1979 Jan-Feb;19(1):81-5. doi: 10.1046/j.1537-2995.1979.19179160273.x.

DOI:10.1046/j.1537-2995.1979.19179160273.x

Abstract

Evaluation of the effects of four suspending media for test cells in antibody detection and identification is reported. The media under investigation are normal saline, low ionic strength salt solution, 22% bovine albumin and polymerized albumin. Hemagglutination at 37 C was enhanced in the test systems employing polymerized albumin. With shortened incubation times, the antiglobulin test was enhanced in tests against red blood cells suspended in the low ionic strength salt solution. When incubation times were extended to 30 minutes, all suspending media were apparently equally effective.

摘要

本文报道了对用于检测细胞抗体的四种悬浮介质的效果评估。所研究的介质为生理盐水、低离子强度盐溶液、22%牛白蛋白和聚合白蛋白。在使用聚合白蛋白的检测系统中，37℃时的血凝反应增强。缩短孵育时间时，针对悬浮于低离子强度盐溶液中的红细胞的抗球蛋白试验增强。当孵育时间延长至30分钟时，所有悬浮介质显然同样有效。