Line J E, Bailey J S, Cox N A, Stern N J, Tompkins T
USDA-ARS, Russell Research Center, Athens, Georgia 30604-5677, USA.
Poult Sci. 1998 Mar;77(3):405-10. doi: 10.1093/ps/77.3.405.
The effect of the yeast, Saccharomyces boulardii, on experimental cecal colonization of broilers with Salmonella typhimurium and Campylobacter jejuni was investigated. Duplicate pens of broiler chicks were given ad libitum access to a standard feed supplemented with no yeast (control), or 1 g (1x), or 100 g (100x) dried S. boulardii/kg feed. All chicks except negative controls were challenged on Day 4 with 3.2 x 10(8) cfu S. typhimurium and 6.5 x 10(8) cfu C. jejuni by oral gavage. After 3 wk, the broilers were euthanatized and ceca were aseptically removed and analyzed for Salmonella and Campylobacter. Frequency of Salmonella colonization was significantly (P < 0.05) reduced due to yeast treatment. Of the positive control birds, 70% were colonized with Salmonella; whereas only 20 and 5% of the 1x and 100x yeast-treated birds were colonized. Mean number of Salmonella per gram of ceca and contents were log 1.64, 0.35, and 0.15, respectively, for the control, 1x, and 100x yeast-treated birds. Campylobacter colonization was not significantly affected by yeast treatment. Similar results were obtained from a second trial conducted in larger isolation floor pens.
研究了酿酒酵母对肉鸡实验性盲肠中鼠伤寒沙门氏菌和空肠弯曲菌定植的影响。将肉鸡雏鸡分成重复的栏，随意给予补充了无酵母（对照）、1克（1倍）或100克（100倍）干酿酒酵母/千克饲料的标准饲料。除阴性对照外，所有雏鸡在第4天通过口服灌喂3.2×10⁸cfu鼠伤寒沙门氏菌和6.5×10⁸cfu空肠弯曲菌进行攻毒。3周后，对肉鸡实施安乐死，无菌取出盲肠并分析其中的沙门氏菌和弯曲菌。由于酵母处理，沙门氏菌定植频率显著降低（P<0.05）。在阳性对照鸡中，70%被沙门氏菌定植；而在1倍和100倍酵母处理的鸡中，分别只有20%和5%被定植。对照、1倍和100倍酵母处理的鸡每克盲肠及其内容物中沙门氏菌的平均数量分别为对数1.64、0.35和0.15。酵母处理对弯曲菌定植没有显著影响。在较大的隔离地面栏中进行的第二项试验也得到了类似结果。
