Oyarzabal O A, Conner D E
Department of Poultry Science, Auburn University, Alabama 36849-5416, USA.
Poult Sci. 1996 Feb;75(2):186-90. doi: 10.3382/ps.0750186.
Providing direct-fed-microbial (DFM) bacteria and fructooligosaccharides (FOS) for the control of potential escalation of Salmonella colonization during simulated feed withdrawal and confinement was assessed. Eight hundred and eighty broilers (16 pens; 55 chicks per pen) were reared to 6 wk of age. Chicks were sprayed with a solution containing 10(6) nalidixic-acid resistant Salmonella typhimuriumNR cells per milliliter on the 2nd d after hatching. Because this first challenge did not yield a high infection rate, chickens were rechallenged per Os at Day 18 by providing water containing 10(7) cells of S. typhimuriumNR per milliliter. At 3 and 5 wk of age, 10 birds per pen were euthanatized and cecal Salmonella were quantified (log colony-forming units per gram). Feed was removed from all pens at 6 wk, and pens were randomly assigned to be either the treatment group or the control group. The treatment groups were provided a DFM (mixture of nine bacteria) and FOS 50 (R) (10%) in the drinking water. The control groups received drinking water only. After 6 h of feed withdrawal, chickens were cooped (eight per coop) and held 10 h. Immediately after confinement, 10 chickens were used for cecal enumeration of S. typhimuriumNR. Salmonella colonization declined from 99% at 3 wk to 44% at 5 wk. After feed withdrawal, application of the treatment, and confinement, 11 and 14% of the treated and control groups, respectively, yielded S. typhimuriumNR by direct plating from ceca (3.87 and 3.75 log 10 cfu/g, respectively). No difference (P > 0.05) in Salmonella colonization occurred between the treated and the control groups; however, enrichment of ceca (incubation in nutrient broth at 37 C for 24 h) yielded a higher incidence of S. typhimuriumNR in the control groups (32% in the treated vs 51% in the control). Ceca weights were greater in the treated group (P < 0.05). Simulated feed withdrawal and confinement did not escalate Salmonella colonization in the chicken ceca.
评估了提供直接投喂微生物（DFM）细菌和低聚果糖（FOS）以控制模拟停喂和圈养期间沙门氏菌定植潜在增加的情况。880只肉鸡（16个围栏；每个围栏55只雏鸡）饲养至6周龄。雏鸡在孵化后第2天喷洒每毫升含10⁶耐萘啶酸鼠伤寒沙门氏菌NR细胞的溶液。由于首次攻毒未产生高感染率，在第18天通过提供每毫升含10⁷鼠伤寒沙门氏菌NR细胞的饮水对鸡再次经口攻毒。在3周龄和5周龄时，每个围栏处死10只鸡，对盲肠沙门氏菌进行定量（每克对数集落形成单位）。在6周龄时从所有围栏中撤走饲料，围栏被随机分配到治疗组或对照组。治疗组在饮水中提供DFM（九种细菌的混合物）和FOS 50（R）（10%）。对照组仅接受饮水。停喂6小时后，将鸡关进鸡笼（每个鸡笼8只）并饲养10小时。圈养后立即使用10只鸡对盲肠中的鼠伤寒沙门氏菌NR进行计数。沙门氏菌定植率从3周龄时的99%降至5周龄时的44%。停喂、施用处理和圈养后，治疗组和对照组分别有11%和14%的鸡通过盲肠直接接种检出鼠伤寒沙门氏菌NR（分别为3.87和3.75 log₁₀ cfu/g）。治疗组和对照组之间沙门氏菌定植没有差异（P>0.05）；然而，盲肠富集培养（在37℃营养肉汤中培养24小时）后，对照组中鼠伤寒沙门氏菌NR的检出率更高（治疗组为32%，对照组为51%）。治疗组的盲肠重量更大（P<0.05）。模拟停喂和圈养并未使鸡盲肠中的沙门氏菌定植增加。
