Ferreira-da-Cruz M F, Galvão-Castro B, Wanke B
Mem Inst Oswaldo Cruz. 1985 Jul-Sep;80(3):301-5. doi: 10.1590/s0074-02761985000300005.
Soluble antigens (Ag) from Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus were prepared and standardized by double immunodiffusion (DID) and immunoelectroosmophoresis (IEOP). No difference in sensitivity was observed between the two techniques; 100% of standard patient sera were positive with P. brasiliensis and A. fumigatus Ag and 83.3% were positive with H. capsulatum Ag. The specificity of the tests was verified testing 96 sera from patients with paracoccidioidomycosis, histoplasmosis, systemic candidiasis, sporotrichosis, tuberculosis, lung cancer, visceral or cutaneous leishmaniasis and 18 sera from healthy individuals. All the three antigens were 100% specific with the DID (using the identification pattern indicated by the confluence of test serum with standard serum precipitin lines as a positive criterium). However in the IEOP, the specificity varied with each Ag. Positive reactions with P. brasiliensis Ag were observed in 16.7% of histoplasmosis sera and in 10% of cutaneous leishmaniasis sera. On the other hand 31.8% of paracoccidioidomycosis and 10% of cutaneous leishmaniasis sera reacted with H. capsulatum Ag. The high sensitivity and specificity of the DID test, its easy reproducibility and low cost, led us to consider it highly appropriate as a routine procedure for the screening of patients with respiratory infections.
制备了巴西副球孢子菌、荚膜组织胞浆菌和烟曲霉的可溶性抗原（Ag），并通过双向免疫扩散（DID）和免疫电渗电泳（IEOP）进行标准化。两种技术在敏感性上未观察到差异；100%的标准患者血清对巴西副球孢子菌和烟曲霉抗原呈阳性反应，83.3%对荚膜组织胞浆菌抗原呈阳性反应。通过检测96份来自副球孢子菌病、组织胞浆菌病、系统性念珠菌病、孢子丝菌病、结核病、肺癌、内脏或皮肤利什曼病患者的血清以及18份健康个体的血清，验证了检测的特异性。在DID中（以检测血清与标准血清沉淀线融合所指示的鉴定模式作为阳性标准），所有三种抗原的特异性均为100%。然而，在IEOP中，每种抗原的特异性各不相同。在16.7%的组织胞浆菌病血清和10%的皮肤利什曼病血清中观察到与巴西副球孢子菌抗原的阳性反应。另一方面，31.8%的副球孢子菌病血清和10%的皮肤利什曼病血清与荚膜组织胞浆菌抗原发生反应。DID检测的高敏感性和特异性、易于重复性和低成本，使我们认为它非常适合作为筛查呼吸道感染患者的常规程序。
