白色念珠菌基因ALS1在酿酒酵母中的表达可诱导其对内皮细胞和上皮细胞的黏附。
Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells.
作者信息
Fu Y, Rieg G, Fonzi W A, Belanger P H, Edwards J E, Filler S G
机构信息
St. John's Cardiovascular Research Center, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 90502, USA.
出版信息
Infect Immun. 1998 Apr;66(4):1783-6. doi: 10.1128/IAI.66.4.1783-1786.1998.
Abstract
To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae. These transformed organisms were screened for adherence to endothelial cells, and a highly adherent clone was identified. The adherence of this clone to endothelial cells was over 100-fold greater than that of control S. cerevisiae transformed with the empty plasmid. This clone also exhibited enhanced adherence to epithelial cells. The C. albicans gene contained within this clone was found to be ALS1. These results indicate that ALS1 may encode a candidal adhesin.
摘要
为了鉴定编码介导白色念珠菌与内皮细胞结合的黏附素的基因，构建了该生物体的基因组文库并用于转化酿酒酵母。筛选这些转化后的生物体与内皮细胞的黏附情况，鉴定出一个高黏附性克隆。该克隆与内皮细胞的黏附力比用空质粒转化的对照酿酒酵母高100多倍。该克隆对上皮细胞的黏附力也增强。发现该克隆中包含的白色念珠菌基因是ALS1。这些结果表明ALS1可能编码一种念珠菌黏附素。
相似文献
1
Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells.白色念珠菌基因ALS1在酿酒酵母中的表达可诱导其对内皮细胞和上皮细胞的黏附。
Infect Immun. 1998 Apr;66(4):1783-6. doi: 10.1128/IAI.66.4.1783-1786.1998.
2
Cloning and characterization of CAD1/AAF1, a gene from Candida albicans that induces adherence to endothelial cells after expression in Saccharomyces cerevisiae.CAD1/AAF1基因的克隆与特性分析，该基因来自白色念珠菌，在酿酒酵母中表达后可诱导其黏附于内皮细胞。
Infect Immun. 1998 May;66(5):2078-84. doi: 10.1128/IAI.66.5.2078-2084.1998.
3
Expression, cloning, and characterization of a Candida albicans gene, ALA1, that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins.白色念珠菌基因ALA1的表达、克隆及特性分析，该基因赋予酿酒酵母对细胞外基质蛋白的黏附特性。
Infect Immun. 1997 Dec;65(12):5289-94. doi: 10.1128/iai.65.12.5289-5294.1997.
4
Candida albicans ALS1: domains related to a Saccharomyces cerevisiae sexual agglutinin separated by a repeating motif.白色念珠菌ALS1：与酿酒酵母性凝集素相关的结构域，由一个重复基序分隔。
Mol Microbiol. 1995 Jan;15(1):39-54. doi: 10.1111/j.1365-2958.1995.tb02219.x.
5
Overexpression of the Candida albicans ALA1 gene in Saccharomyces cerevisiae results in aggregation following attachment of yeast cells to extracellular matrix proteins, adherence properties similar to those of Candida albicans.白色念珠菌的ALA1基因在酿酒酵母中的过表达导致酵母细胞附着于细胞外基质蛋白后发生聚集，其黏附特性与白色念珠菌相似。
Infect Immun. 1999 Nov;67(11):6040-7. doi: 10.1128/IAI.67.11.6040-6047.1999.
6
Candida albicans Als1p: an adhesin that is a downstream effector of the EFG1 filamentation pathway.白色念珠菌Als1p：一种粘附素，是EFG1丝状化途径的下游效应物。
Mol Microbiol. 2002 Apr;44(1):61-72. doi: 10.1046/j.1365-2958.2002.02873.x.
7
Identification of Candida albicans genes that induce Saccharomyces cerevisiae cell adhesion and morphogenesis.白色念珠菌中诱导酿酒酵母细胞黏附和形态发生的基因的鉴定。
Biotechnol Prog. 2005 Nov-Dec;21(6):1601-9. doi: 10.1021/bp050236c.
8
Adherence of clinical isolates of Saccharomyces cerevisiae to buccal epithelial cells.酿酒酵母临床分离株对颊上皮细胞的黏附
Med Mycol. 2001 Feb;39(1):123-7. doi: 10.1080/mmy.39.1.123.127.
9
Many of the genes required for mating in Saccharomyces cerevisiae are also required for mating in Candida albicans.酿酒酵母中交配所需的许多基因在白色念珠菌交配时也同样需要。
Mol Microbiol. 2002 Dec;46(5):1345-51. doi: 10.1046/j.1365-2958.2002.03263.x.
10
Complementary adhesin function in C. albicans biofilm formation.白色念珠菌生物膜形成中的互补黏附素功能。
Curr Biol. 2008 Jul 22;18(14):1017-24. doi: 10.1016/j.cub.2008.06.034.
引用本文的文献
1
Increased fungal burden in the gastrointestinal tract of brain-dead organ donors.脑死亡器官捐献者胃肠道中真菌负荷增加。
Microbiol Spectr. 2025 Aug 5;13(8):e0334124. doi: 10.1128/spectrum.03341-24. Epub 2025 Jun 18.
2
Adhesins in the virulence of opportunistic fungal pathogens of human.黏附素在人类机会性真菌病原体毒力中的作用
Mycology. 2021 Jul 5;12(4):296-324. doi: 10.1080/21501203.2021.1934176. eCollection 2021.
3
Ozone ultrafine bubble water inhibits the early formation of Candida albicans biofilms.臭氧超细微气泡水抑制白念珠菌生物膜的早期形成。
PLoS One. 2021 Dec 10;16(12):e0261180. doi: 10.1371/journal.pone.0261180. eCollection 2021.
4
Comprehensive genetic analysis of adhesin proteins and their role in virulence of Candida albicans.黏附素蛋白的综合遗传分析及其在白念珠菌毒力中的作用。
Genetics. 2021 Feb 9;217(2). doi: 10.1093/genetics/iyab003.
5
Bgl2p, Ecm33p, and Als1p proteins are involved in adhesion to saliva-coated hydroxyapatite.Bgl2p、Ecm33p和Als1p蛋白参与对唾液包被的羟基磷灰石的黏附。
J Oral Microbiol. 2021 Feb 12;13(1):1879497. doi: 10.1080/20002297.2021.1879497.
6
Activation of EphA2-EGFR signaling in oral epithelial cells by Candida albicans virulence factors.口腔上皮细胞中白色念珠菌毒力因子对 EphA2-EGFR 信号的激活作用。
PLoS Pathog. 2021 Jan 20;17(1):e1009221. doi: 10.1371/journal.ppat.1009221. eCollection 2021 Jan.
7
An Amyloid Core Sequence in the Major Candida albicans Adhesin Als1p Mediates Cell-Cell Adhesion.主要白色念珠菌黏附素 Als1p 中的淀粉样核心序列介导细胞-细胞黏附。
mBio. 2019 Oct 8;10(5):e01766-19. doi: 10.1128/mBio.01766-19.
8
Pathogen interactions with endothelial cells and the induction of innate and adaptive immunity.病原体与内皮细胞的相互作用及固有和适应性免疫的诱导。
Eur J Immunol. 2018 Oct;48(10):1607-1620. doi: 10.1002/eji.201646789. Epub 2018 Sep 21.
9
What We Do Not Know about Fungal Cell Adhesion Molecules.我们对真菌细胞粘附分子的未知之处。
J Fungi (Basel). 2018 May 17;4(2):59. doi: 10.3390/jof4020059.
10
Community Development between and Mediated by InlJ and Als3.InlJ 和 Als3 介导的 之间的社区发展。
mBio. 2018 Apr 24;9(2):e00202-18. doi: 10.1128/mBio.00202-18.
本文引用的文献
1
Cloning and characterization of CAD1/AAF1, a gene from Candida albicans that induces adherence to endothelial cells after expression in Saccharomyces cerevisiae.CAD1/AAF1基因的克隆与特性分析，该基因来自白色念珠菌，在酿酒酵母中表达后可诱导其黏附于内皮细胞。
Infect Immun. 1998 May;66(5):2078-84. doi: 10.1128/IAI.66.5.2078-2084.1998.
2
Expression, cloning, and characterization of a Candida albicans gene, ALA1, that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins.白色念珠菌基因ALA1的表达、克隆及特性分析，该基因赋予酿酒酵母对细胞外基质蛋白的黏附特性。
Infect Immun. 1997 Dec;65(12):5289-94. doi: 10.1128/iai.65.12.5289-5294.1997.
3
Candida albicans stimulates cytokine production and leukocyte adhesion molecule expression by endothelial cells.白色念珠菌可刺激内皮细胞产生细胞因子并表达白细胞黏附分子。
Infect Immun. 1996 Jul;64(7):2609-17. doi: 10.1128/iai.64.7.2609-2617.1996.
4
Cell surface anchorage and ligand-binding domains of the Saccharomyces cerevisiae cell adhesion protein alpha-agglutinin, a member of the immunoglobulin superfamily.酿酒酵母细胞粘附蛋白α-凝集素的细胞表面锚定域和配体结合域，免疫球蛋白超家族的成员。
Mol Cell Biol. 1993 Apr;13(4):2554-63. doi: 10.1128/mcb.13.4.2554-2563.1993.
5
Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure.采用LiAc/SS-DNA/PEG方法对完整酵母细胞进行转化的研究。
Yeast. 1995 Apr 15;11(4):355-60. doi: 10.1002/yea.320110408.
6
Candida albicans ALS1: domains related to a Saccharomyces cerevisiae sexual agglutinin separated by a repeating motif.白色念珠菌ALS1：与酿酒酵母性凝集素相关的结构域，由一个重复基序分隔。
Mol Microbiol. 1995 Jan;15(1):39-54. doi: 10.1111/j.1365-2958.1995.tb02219.x.
7
Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.源自脐静脉的人内皮细胞培养。通过形态学和免疫学标准进行鉴定。
J Clin Invest. 1973 Nov;52(11):2745-56. doi: 10.1172/JCI107470.
8
AG alpha 1 is the structural gene for the Saccharomyces cerevisiae alpha-agglutinin, a cell surface glycoprotein involved in cell-cell interactions during mating.AG alpha 1是酿酒酵母α-凝集素的结构基因，α-凝集素是一种细胞表面糖蛋白，在交配过程中参与细胞间相互作用。
Mol Cell Biol. 1989 Aug;9(8):3155-65. doi: 10.1128/mcb.9.8.3155-3165.1989.
9
Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.λ YES：一种用于通过酵母和大肠杆菌突变互补来分离基因的多功能cDNA表达载体。
Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1731-5. doi: 10.1073/pnas.88.5.1731.
10
Dominant genetics using a yeast genomic library under the control of a strong inducible promoter.在强诱导型启动子控制下，利用酵母基因组文库进行显性遗传学研究。
Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11589-93. doi: 10.1073/pnas.89.23.11589.
Zotero 插件