Stockton B M, Cheng G, Manjunath N, Ardman B, von Andrian U H
Department of Pathology, Tufts University, Boston, Massachusetts 02111, USA.
Immunity. 1998 Mar;8(3):373-81. doi: 10.1016/s1074-7613(00)80542-7.
We report that the cell surface mucin CD43 acts as an anti-adhesin on T lymphocytes. CD43-deficient murine lymphocytes homed significantly more frequently to secondary lymphoid organs than wild-type cells. Intravital microscopy of peripheral lymph node venules revealed that CD43-deficient lymphocytes were twice as likely to tether, roll, and stick than wild-type cells. This effect was due to CD43 interference with the homing receptor, L-selectin, and was most pronounced in venules with low L-selectin ligand density. In vitro, CD43-deficient cells tethered to L-selectin ligands more efficiently and rolled more slowly than wild-type lymphocytes. Thus, CD43 exerts a negative regulatory effect on T cell trafficking by counterbalancing L-selectin-mediated adhesion.
我们报告称，细胞表面黏蛋白CD43在T淋巴细胞上作为一种抗黏附分子发挥作用。与野生型细胞相比，缺乏CD43的小鼠淋巴细胞归巢至次级淋巴器官的频率显著更高。对外周淋巴结小静脉进行的活体显微镜观察显示，缺乏CD43的淋巴细胞与野生型细胞相比，拴系、滚动和黏附的可能性高出两倍。这种效应是由于CD43干扰归巢受体L-选择素所致，并且在L-选择素配体密度低的小静脉中最为明显。在体外，缺乏CD43的细胞比野生型淋巴细胞更有效地拴系于L-选择素配体，且滚动得更慢。因此，CD43通过平衡L-选择素介导的黏附作用，对T细胞迁移发挥负向调节作用。
