Sela M, Mozes E
Biochimie. 1976;58(1-2):173-7. doi: 10.1016/s0300-9084(76)80367-7.
The effect of the double-stranded synthetic polynucleotide, poly(A).poly(U), on the immune response of inbred mouse strains to multichain synthetic polypeptides was studied. Poly(A).poly(U) did not affect immune responses controlled by H-2 linked genes. Thus, when either (T,G)-A- -L or (Phe,G)-A--L were injected into high or low responder mice followed by administration of poly(A).poly(U) 24 h after immunization, no increase in the antibody titers was observed. In contrast, poly(A).poly(U) increased significantly the response to polyproline, which is controlled by a non H-2 linked gene, in low responder mice. However, the polyribonucleotide had no effect on the antibody titers of the SJL mice, the high responders to multichain polyproline. When poly(A).poly(U) was injected into DBA/1 mice following immunization with (Phe,G)-Pro- -L, the polynucleotide enhanced the low response to the Pro- -l region at the expense of the anti (Phe,G) response which is normally high in this mouse strain. In this case poly(A).poly(U) caused an intramolecular antigenic competition. The general conclusion of this study is that the chemical nature of the antigenic determinant plays an important role in determining the type of influence exerted by poly(A).poly(U).
研究了双链合成多核苷酸聚（A）·聚（U）对近交系小鼠品系对多链合成多肽免疫反应的影响。聚（A）·聚（U）不影响由H - 2连锁基因控制的免疫反应。因此，当将（T，G）-A--L或（苯丙氨酸，G）-A--L注入高反应或低反应小鼠，然后在免疫后24小时给予聚（A）·聚（U）时，未观察到抗体滴度增加。相反，聚（A）·聚（U）显著增强了低反应小鼠对由非H - 2连锁基因控制的聚脯氨酸的反应。然而，该多聚核苷酸对多链聚脯氨酸的高反应者SJL小鼠的抗体滴度没有影响。当在用（苯丙氨酸，G）-Pro--L免疫DBA/1小鼠后注射聚（A）·聚（U）时，该多核苷酸增强了对Pro--l区域的低反应，代价是该小鼠品系中通常较高的抗（苯丙氨酸，G）反应。在这种情况下，聚（A）·聚（U）引起了分子内抗原竞争。本研究的总体结论是，抗原决定簇的化学性质在确定聚（A）·聚（U）施加的影响类型方面起着重要作用。
