Fu Yawei, Liang Xiaoxiao, Li Donghua, Gao Hu, Wang Yadong, Li Wenting, Xu Kang, Hu Fangzhou
CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China.
Front Nutr. 2021 Jun 17;8:676035. doi: 10.3389/fnut.2021.676035. eCollection 2021.
Tryptophan (Trp) has received increasing attention in the maintenance of intestinal function. In this study, improved triploid crucian carp (ITCC) fed diets containing 6.35 g kg Trp had higher average daily gain (ADG) and improved villus height (VH) and crypt depth (CD) in the intestine compared to the control group. To elucidate the potential mechanisms, we used RNA sequencing (RNA-seq) to investigate changes in the intestinal transcriptome and 16S rRNA gene sequencing to measure the intestinal microbiota in response to 6.35 g kg Trp feeding in ITCC. Dietary Trp altered intestinal gene expression involved in nutrient transport and metabolism. Differentially expressed transcripts (DETs) were highly enriched in key pathways containing protein digestion and absorption and the AMPK signaling pathway. 16S rRNA sequencing showed that 6.35 g kg Trp significantly increased the abundance of the genus , and the Firmicutes/Bacteroidetes ratio at the phylum level ( < 0.05). In addition, bacterial richness indices (Simpson index) significantly increased ( < 0.05) community evenness in response to 6.35 g kg Trp. In conclusion, appropriate dietary Trp improves the growth performance, and influences the intestinal flora of ITCC. This study might be helpful to guide the supply of dietary exogenous Trp in ITCC breeding.
色氨酸（Trp）在维持肠道功能方面受到越来越多的关注。在本研究中，与对照组相比，饲喂含6.35 g/kg色氨酸日粮的改良三倍体鲫鱼（ITCC）具有更高的平均日增重（ADG），且肠道绒毛高度（VH）和隐窝深度（CD）得到改善。为阐明潜在机制，我们使用RNA测序（RNA-seq）研究肠道转录组的变化，并使用16S rRNA基因测序来测量ITCC中饲喂6.35 g/kg色氨酸后肠道微生物群的变化。日粮色氨酸改变了参与营养物质运输和代谢的肠道基因表达。差异表达转录本（DETs）在包含蛋白质消化吸收和AMPK信号通路的关键途径中高度富集。16S rRNA测序表明，6.35 g/kg色氨酸显著增加了属的丰度，以及门水平上的厚壁菌门/拟杆菌门比率（P<0.05）。此外，细菌丰富度指数（辛普森指数）显著增加（P<0.05），响应6.35 g/kg色氨酸的群落均匀度也增加。总之，适宜的日粮色氨酸可改善ITCC的生长性能，并影响其肠道菌群。本研究可能有助于指导ITCC养殖中日粮外源色氨酸的供应。
