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本文引用的文献

1
Elimination of Differences in the Mobility of Flax Isoperoxidases on PAGE by Digestion with alpha-Mannosidase.用α-甘露糖苷酶消化消除亚麻异过氧化物酶在聚丙烯酰胺凝胶电泳上迁移率的差异
Plant Physiol. 1988 Jan;86(1):288-92. doi: 10.1104/pp.86.1.288.
2
Purification of horse-radish peroxidase and comparison of its properties with those of catalase and methaemoglobin.辣根过氧化物酶的纯化及其与过氧化氢酶和高铁血红蛋白性质的比较。
Biochem J. 1951 Jun;49(1):88-104. doi: 10.1042/bj0490088.
3
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
4
Isolation, characterization and expression of cationic peroxidase isozymes released into the medium of cultured tobacco cells.培养烟草细胞培养基中释放的阳离子过氧化物酶同工酶的分离、特性鉴定及表达
Eur J Biochem. 1995 Mar 15;228(3):855-62.
5
Rate enhancement of compound I formation of barley peroxidase by ferulic acid, caffeic acid, and coniferyl alcohol.阿魏酸、咖啡酸和松柏醇对大麦过氧化物酶化合物I形成的速率增强作用。
Biochemistry. 1995 Mar 28;34(12):4022-9. doi: 10.1021/bi00012a021.
6
Purification and partial characterization of 'extensin peroxidase'.“伸展蛋白过氧化物酶”的纯化及部分特性分析
Biochem Soc Trans. 1995 May;23(2):154S. doi: 10.1042/bst023154s.
7
Calcium requirement for the secretion of peroxidases by plant cell suspensions.植物细胞悬浮液分泌过氧化物酶所需的钙
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8
[Isolation, purification and physico- chemical properties of turnip peroxidases].[芜菁过氧化物酶的分离、纯化及理化性质]
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9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.从电转印到聚偏二氟乙烯膜上的皮摩尔量蛋白质中获得的序列。
J Biol Chem. 1987 Jul 25;262(21):10035-8.

铁刀木植物细胞培养物中过氧化物酶的纯化、部分特性鉴定及生物转化研究

Purification and partial characterization of a peroxidase from plant cell cultures of Cassia didymobotrya and biotransformation studies.

作者信息

Vitali A, Botta B, Delle Monache G, Zappitelli S, Ricciardi P, Melino S, Petruzzelli R, Giardina B

机构信息

C.N.R. Centro Chimica dei Recettori e delle Molecole Biologicamente Attive, Istituto di Chimica e Chimica Clinica, Facoltà di Medicina e Chirurgia, Università Cattolica S. Cuore, L.go F. Vito 1, 00168 Roma, Italy.

出版信息

Biochem J. 1998 Apr 15;331 ( Pt 2)(Pt 2):513-9. doi: 10.1042/bj3310513.

DOI:10.1042/bj3310513
PMID:9531492
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1219383/
Abstract

An acidic peroxidase (EC 1.11.1.7) produced by cell suspension cultures of Cassia didymobotrya (wild senna) was purified from culture medium collected on the 29th day. The enzyme was shown to be a glycoprotein with a pI of 3.5, a molecular mass of approx. 43 kDa by SDS/PAGE and 50 kDa by gel filtration. The N-terminal sequence was very similar to those of other plant peroxidases. The peroxidase was characterized by a high specificity towards coniferyl alcohol and other natural phenolics such as guaiacol and ferulic and caffeic acids. These findings suggest that the enzyme is involved in lignification processes of the cell wall. Moreover, the enzyme was able to catalyse the oxidation of 4,3',4'-trihydroxychalcone and 4, 3',4'-trihydroxy-3-methoxychalcone to the corresponding 3, 3'-biflavanones, as mixtures of racemic and meso forms.

摘要

从第29天收集的培养基中纯化出由双荚决明（野生番泻叶）细胞悬浮培养物产生的一种酸性过氧化物酶（EC 1.11.1.7）。该酶被证明是一种糖蛋白，其pI为3.5，通过SDS/PAGE测得分子量约为43 kDa，通过凝胶过滤测得分子量为50 kDa。其N端序列与其他植物过氧化物酶的序列非常相似。该过氧化物酶对松柏醇和其他天然酚类物质（如愈创木酚、阿魏酸和咖啡酸）具有高度特异性。这些发现表明该酶参与细胞壁的木质化过程。此外，该酶能够将4,3',4'-三羟基查耳酮和4,3',4'-三羟基-3-甲氧基查耳酮催化氧化为相应的3,3'-双黄酮，以外消旋体和内消旋体形式的混合物存在。