Viacava P, Naccarato A G, Bevilacqua G
Department of Oncology, Pisa, Italy.
Virchows Arch. 1998 Mar;432(3):255-60. doi: 10.1007/s004280050163.
GCDFP-15, a glycoprotein identified in the cyst fluid of cystic breast disease, is considered to be a marker of apocrine differentiation. Studies on GCDFP-15 localization in adult normal tissues are lacking, and no information on GCDFP-15 expression during fetal development has been reported. We investigated GCDFP-15 expression in a large series of formalin-fixed, paraffin-embedded normal human adult and fetal tissues using the monoclonal antibody BRST-2. In normal adult tissues GCDFP-15 expression was found in all apocrine, lacrimal, ceruminous and Moll's glands and in numerous serous cells of the submandibular, sublingual and minor salivary glands. The serous cells of nasal and bronchial glands were also positive; parotid and laryngeal glands showed rare immunoreactive cells. GCDFP-15-positive cells were observed in all cutaneous eccrine glands from different body sites. In fetal tissues immunoreactivity was observed in numerous acinous cells of all tracheal, bronchial and submandibular salivary glands. GCDFP-15 positivity was identified in numerous cells of all axillary sweat glands and in rare cells of some sweat glands of the thorax, abdomen, back, leg and arm. In both apocrine and nonapocrine glands GCDFP-15 was always localized in the secretory component. These data suggest that GCDFP-15 is a glandular differentiation marker associated with apocrine secretion; that it is expressed in glands that have phylogenetic origins in common with apocrine glands (submandibular salivary and submucosal bronchial glands); and that eccrine cutaneous glands express GCDFP-15 and thus might be referred to as mixed apocrine-eccrine glands. GCDFP-15 is expressed during fetal development and may represent a common marker of embryologically linked glandular structures.
GCDFP-15是一种在乳腺囊性疾病囊液中发现的糖蛋白，被认为是顶泌汗腺分化的标志物。目前缺乏关于GCDFP-15在成人正常组织中定位的研究，也没有关于其在胎儿发育过程中表达情况的报道。我们使用单克隆抗体BRST-2，对大量福尔马林固定、石蜡包埋的正常成人和胎儿组织中GCDFP-15的表达进行了研究。在正常成人组织中，GCDFP-15表达见于所有顶泌汗腺、泪腺、耵聍腺和睑缘腺，以及下颌下腺、舌下腺和小唾液腺的许多浆液细胞。鼻腺和支气管腺的浆液细胞也呈阳性；腮腺和喉腺可见罕见的免疫反应性细胞。在来自不同身体部位的所有皮肤外分泌腺中均观察到GCDFP-15阳性细胞。在胎儿组织中，在所有气管、支气管和下颌下唾液腺的许多腺泡细胞中观察到免疫反应性。在所有腋窝汗腺的许多细胞以及胸部、腹部、背部、腿部和手臂的一些汗腺的罕见细胞中鉴定出GCDFP-15阳性。在顶泌汗腺和非顶泌汗腺中，GCDFP-15总是定位于分泌成分中。这些数据表明，GCDFP-15是一种与顶泌汗腺分泌相关的腺分化标志物；它在与顶泌汗腺有共同系统发育起源的腺体（下颌下唾液腺和黏膜下支气管腺）中表达；皮肤外分泌腺表达GCDFP-15，因此可能被称为混合顶泌-外分泌腺。GCDFP-15在胎儿发育过程中表达，可能代表胚胎学上相关腺体结构的共同标志物。
