Queiroz-da-Silva L H, De-Franco M, Sant'Anna O A
Departamento de Apoio, Curso de Medicina Veterinária, Universidade Estadual Paulista, Araçatuba, SP, Brasil.
Braz J Med Biol Res. 1997 Nov;30(11):1309-13. doi: 10.1590/s0100-879x1997001100008.
Innate and acquired resistance to rabies infection was investigated in mice genetically selected for high (H) or low (L) antibody responsiveness from selections I, III and IV and in mice selected for maximal (AIRmax) or minimal (AIRmin) acute inflammatory reaction. These mouse lines were infected intramuscularly with different virus dilutions and the LD50 was determined. The HIII and HIV mouse lines were more susceptible than the LIII and LIV lines and the HI line showed a discrete but higher resistance than the LI line. Analysis of the interline (H x L) F1 hybrids from selections III and IV indicated different dominance effects on the "resistant" and "susceptible" phenotypes when the route of vaccination was changed. No differences were observed between the AIRmax and AIRmin mice, suggesting that inflammation plays a minor role in the resistance to rabies virus. The comparison of LD50 in mice vaccinated by distinct routes showed that the highest interline difference occurred after intramuscular vaccination (250-fold between H and L and 800-fold between F1 and L). These results indicate that different mechanisms may participate in acquired antirabies resistance.
对从选择I、III和IV中选出的具有高（H）或低（L）抗体反应性的小鼠，以及选择出的具有最大（AIRmax）或最小（AIRmin）急性炎症反应的小鼠，研究了其对狂犬病感染的先天和后天抗性。这些小鼠品系通过肌肉注射不同病毒稀释液进行感染，并测定半数致死剂量（LD50）。HIII和HIV小鼠品系比LIII和LIV品系更易感，并且HI品系显示出比LI品系有明显但更高的抗性。对来自选择III和IV的品系间（H×L）F1杂种的分析表明，当改变疫苗接种途径时，对“抗性”和“易感”表型有不同的显性效应。在AIRmax和AIRmin小鼠之间未观察到差异，这表明炎症在对狂犬病病毒的抗性中起次要作用。通过不同途径接种疫苗的小鼠中LD50的比较表明，肌肉注射疫苗后品系间差异最大（H和L之间为250倍，F1和L之间为800倍）。这些结果表明，不同机制可能参与后天抗狂犬病抗性。
