De Franco M, Massa S, Vassão R C, Siqueira M, Sant'Anna O A
Laboratório de Imunogenética, Instituto Butantan, São Paulo, Brazil.
Arch Virol. 1996;141(8):1397-406. doi: 10.1007/BF01718243.
The amplification of "high" (H) and "low" (L) multispecific antibody responses achieved respectively by H and L lines of selection GP represents a valuable tool in the genetic study of host-infection interactions. These lines were obtained by bidirectional selective breeding for high (HGP) or low (LGP) antibody production to natural complex antigens. HGP and LGP parental lines and reciprocal F1 hybrids, as well as their F2 segregants and backcrosses were submitted to immunization and challenge with rabies virus CVS strain. Acquired resistance was 1000-fold higher in HGP than LGP mice, with a dominance effect to low antibody production observed in F1 hybrids. An association between high antibody response and acquired resistance (P < 0.001) in F2 segregant mice was noticed. The genetic study was performed in these several populations, with a single dose of 104.5-fold LD50 CVS. We could demonstrate 3 independent loci regulating the anti-rabies antibody production, that are distinct, at least in part, from the 10 genes controlling the antigen selection response (sheep erythrocytes) of selection GP.
通过选择系GP的H系和L系分别实现的“高”（H）和“低”多特异性抗体反应的增强，是宿主-感染相互作用基因研究中的一种有价值的工具。这些品系是通过对天然复合抗原产生高（HGP）或低（LGP）抗体的双向选择性育种获得的。HGP和LGP亲本品系以及正反交F1杂种，以及它们的F2分离株和回交后代，均接受狂犬病病毒CVS株的免疫和攻击。HGP小鼠获得性抵抗力比LGP小鼠高1000倍，在F1杂种中观察到对低抗体产生的显性效应。在F2分离小鼠中发现高抗体反应与获得性抵抗力之间存在关联（P<0.001）。在这些几个群体中进行了基因研究，使用单剂量104.5倍LD50的CVS。我们能够证明有3个独立的基因座调节抗狂犬病抗体的产生，这些基因座至少部分不同于控制选择系GP的抗原选择反应（绵羊红细胞）的10个基因。
