Kyushiki H, Kuga Y, Suzuki M, Takahashi E, Horie M
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co. Ltd., Tokushima, Japan.
Cytogenet Cell Genet. 1997;79(1-2):114-7. doi: 10.1159/000134695.
The RING-finger is a unique zinc-chelating domain involved in mediating protein-protein interactions. The extensive sequence homology within the RING-finger domain allowed us to clone a novel member of the RING-finger family of genes. This cDNA clone, designated RNF5 (Ring-finger protein 5), contained an open reading frame of 540 nucleotides. Its predicted amino acid sequence revealed significant homology to a hypothetical protein encoded by Caenorhabditis elegans cosmid C16C10.7. The expression of RNF5 was detected in a variety of human tissues. The RNF5 gene was mapped by fluorescence in situ hybridization to chromosome 6p21.31. Radiation hybrid mapping further assigned RNF5 to a region proximal to the major histocompatibility complex (MHC) on chromosome 6. RNF5 is the third RING-finger gene identified in the region proximal to MHC raising the possibility that the RING-finger family of genes may exist as a cluster in this region.
环状结构域是一种独特的锌螯合结构域，参与介导蛋白质-蛋白质相互作用。环状结构域内广泛的序列同源性使我们能够克隆环状结构域基因家族的一个新成员。这个cDNA克隆被命名为RNF5（环状结构域蛋白5），包含一个540个核苷酸的开放阅读框。其预测的氨基酸序列与秀丽隐杆线虫粘粒C16C10.7编码的一种假定蛋白具有显著同源性。在多种人类组织中检测到了RNF5的表达。通过荧光原位杂交将RNF5基因定位到6号染色体p21.31。辐射杂种图谱进一步将RNF5定位到6号染色体上主要组织相容性复合体（MHC）近端的一个区域。RNF5是在MHC近端区域鉴定出的第三个环状结构域基因，这增加了环状结构域基因家族可能在该区域以簇的形式存在的可能性。
