Scarpato R, Lori A, Panasiuk G, Barale R
Dipartimento di Scienze dell'Uomo e dell'Ambiente, Università di Pisa, Italy.
Cytogenet Cell Genet. 1997;79(1-2):153-6. doi: 10.1159/000134708.
Using whole-chromosome painting probes, we have analyzed the frequency of translocations of chromosomes 1, 3, and 10 in peripheral lymphocytes of 20 Gomel (Belarus) children, including both thyroid tumor affected and healthy individuals. Gomel was one of the most heavily radiocontaminated areas due to fallout from the Chernobyl nuclear power plant disaster. As controls, 14 healthy children from Pisa (Italy) were investigated simultaneously. Translocation rates were significantly higher in the tumor affected (1.71 +/- 0.68) and healthy Gomel children (2.69 +/- 0.50) than in the Italian controls (0.79 +/- 0.24). We also observed, in healthy Gomel children, an approximately three-fold higher frequency of chromosome 10 translocations compared to translocations affecting chromosomes 1 or 3 (P = 0.0096), a difference that was made even larger (about four fold) after correcting for chromosome size (P = 0.0009). This finding suggests a preferential involvement of chromosome 10 in translocations induced in vivo by low levels of ionizing radiation.
我们使用全染色体涂染探针，分析了20名来自白俄罗斯戈梅利的儿童外周血淋巴细胞中1号、3号和10号染色体的易位频率，这些儿童既有甲状腺肿瘤患者，也有健康个体。由于切尔诺贝利核电站灾难产生的放射性尘埃，戈梅利是受放射性污染最严重的地区之一。作为对照，我们同时对14名来自意大利比萨的健康儿童进行了调查。肿瘤患儿（1.71±0.68）和戈梅利健康儿童（2.69±0.50）的易位率显著高于意大利对照组儿童（0.79±0.24）。我们还观察到，在戈梅利健康儿童中，10号染色体易位的频率比1号或3号染色体易位的频率高出约三倍（P = 0.0096），在根据染色体大小校正后，这一差异变得更大（约四倍）（P = 0.0009）。这一发现表明，10号染色体在低水平电离辐射体内诱导的易位中更容易受到影响。
