De Pedro N, Pinillos M L, Valenciano A I, Alonso-Bedate M, Delgado M J
Departamento de Biología Animal II (Fisiología Animal), Facultad de Ciencias Biológicas, Universidad Complutense, Madrid, Spain.
Peptides. 1998;19(3):505-11. doi: 10.1016/s0196-9781(97)00469-5.
The possible action of 5-HT on feeding behavior in goldfish has been studied. Food intake was significantly reduced by intracerebroventricular (ICV) injection of serotonin (5-HT, 10 microg) at 2 h postinjection. After peripheral (intraperitoneal) administration of 5-HT (1 and 10 microg/g bw), no significant modifications in food intake were detected. Thus, it can be concluded that there is a central anoretic action of 5-HT in teleost fish. Taking in mind the inhibitory effect of corticotropin releasing factor (CRF) on feeding in teleosts and the interactions between 5-HT and CRF described in mammals, we investigated the possible involvement of CRF as mediator of the 5-HT anoretic action in goldfish. The ICV pretreatment with alpha-Helical CRF[9-41](20 microg) partially blocked the inhibitory effect of 5-HT on food consumption in goldfish. These results show that CRF mediates, at least in part, the 5-HT-induced feeding inhibition in goldfish. On the other hand, the alterations in hypothalamic indoleamines content evoked by ICV treatments would suggest that the activation of CRF neurons by 5-HT appears to inhibit hypothalamic serotoninergic transmission, supporting the intermediate role of this neuropeptide in the central anoretic effect of 5-HT in goldfish.
人们已经对5-羟色胺（5-HT）对金鱼摄食行为的可能作用进行了研究。脑室内（ICV）注射血清素（5-HT，10微克）后2小时，食物摄入量显著减少。外周（腹腔内）给予5-HT（1和10微克/克体重）后，未检测到食物摄入量有显著变化。因此，可以得出结论，5-HT在硬骨鱼中具有中枢性厌食作用。考虑到促肾上腺皮质激素释放因子（CRF）对硬骨鱼摄食的抑制作用以及哺乳动物中描述的5-HT与CRF之间的相互作用，我们研究了CRF作为金鱼中5-HT厌食作用介质的可能参与情况。用α-螺旋CRF[9-41]（20微克）进行脑室内预处理部分阻断了5-HT对金鱼食物消耗的抑制作用。这些结果表明，CRF至少部分介导了5-HT诱导的金鱼摄食抑制。另一方面，脑室内处理引起的下丘脑吲哚胺含量变化表明，5-HT对CRF神经元的激活似乎抑制了下丘脑5-羟色胺能传递，支持了这种神经肽在金鱼5-HT中枢性厌食作用中的中间作用。
