酿酒酵母液泡膜囊泡通过质子梯度驱动摄取镍。
Proton gradient-driven nickel uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae.
作者信息
Nishimura K, Igarashi K, Kakinuma Y
机构信息
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
出版信息
J Bacteriol. 1998 Apr;180(7):1962-4. doi: 10.1128/JB.180.7.1962-1964.1998.
Abstract
A vacuolar H+-ATPase-negative mutant of Saccharomyces cerevisiae was highly sensitive to nickel ion. Accumulation of nickel ion in the cells of this mutant of less than 60% of the value for the parent strain arrested growth, suggesting a role for this ATPase in sequestering nickel ion into vacuoles. An artificially imposed pH gradient (interior acid) induced transient nickel ion uptake by vacuolar membrane vesicles, which was inhibited by collapse of the pH difference but not of the membrane potential. Nickel ion transport into vacuoles in a pH gradient-dependent manner is thus important for its detoxification in yeast.
摘要
酿酒酵母的一种液泡H⁺-ATP酶阴性突变体对镍离子高度敏感。该突变体细胞中镍离子的积累量不到亲本菌株的60%时就会停止生长，这表明这种ATP酶在将镍离子隔离到液泡中发挥作用。人为施加的pH梯度（内部呈酸性）会诱导液泡膜囊泡短暂摄取镍离子，pH差异的消失会抑制这种摄取，但膜电位的消失不会。因此，镍离子以pH梯度依赖的方式转运到液泡中对其在酵母中的解毒很重要。
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