Hunt G J, Guzmán-Novoa E, Fondrk M K, Page R E
Department of Entomology, Purdue University, West Lafayette, Indiana 47907-1158, USA.
Genetics. 1998 Mar;148(3):1203-13. doi: 10.1093/genetics/148.3.1203.
A study was conducted to identify quantitative trait loci (QTLs) that affect colony-level stinging behavior and individual body size of honey bees. An F1 queen was produced from a cross between a queen of European origin and a drone descended from an African subspecies. Haploid drones from the hybrid queen were individually backcrossed to sister European queens to produce 172 colonies with backcross workers that were evaluated for tendency to sting. Random amplified polymorphic DNA markers were scored from the haploid drone fathers of these colonies. Wings of workers and drones were used as a measure of body size because Africanized bees in the Americas are smaller than European bees. Standard interval mapping and multiple QTL models were used to analyze data. One possible QTL was identified with a significant effect on tendency to sting (LOD 3.57). Four other suggestive QTLs were also observed (about LOD 1.5). Possible QTLs also were identified that affect body size and were unlinked to defensive-behavior QTLs. Two of these were significant (LOD 3.54 and 5.15).
开展了一项研究，以确定影响蜜蜂群体水平蜇刺行为和个体体型的数量性状基因座（QTL）。一只F1蜂王由一只欧洲血统的蜂王与一只源自非洲亚种的雄蜂杂交产生。来自杂交蜂王的单倍体雄蜂分别与欧洲蜂王姐妹回交，以产生172个群体，其回交工蜂被评估蜇刺倾向。从这些群体的单倍体雄蜂父亲中对随机扩增多态性DNA标记进行评分。工蜂和雄蜂的翅膀被用作体型的衡量指标，因为美洲的非洲化蜜蜂比欧洲蜜蜂体型小。使用标准区间作图和多QTL模型分析数据。确定了一个对蜇刺倾向有显著影响的可能QTL（LOD 3.57）。还观察到其他四个暗示性QTL（约LOD 1.5）。也确定了影响体型且与防御行为QTL不连锁的可能QTL。其中两个是显著的（LOD 3.54和5.15）。
