Antón R, Puig L, Esgleyes T, de Moragas J M, Vila L
Laboratory of Inflammation Mediators, Institute of Research, Barcelona, Spain.
J Invest Dermatol. 1998 Apr;110(4):303-10. doi: 10.1046/j.1523-1747.1998.00159.x.
We recently found that normal human epidermis produces relatively high amounts of hepoxilins and trioxilins in vitro. Therefore, the aim of this study was to demonstrate the presence of these compounds in psoriatic lesions. Extracts from scales of patients with chronic stable plaque psoriasis were analyzed by a combination of high performance liquid chromatography and gas chromatography-mass spectrometry techniques. We found that the levels of hepoxilin B3 were more than 16-fold higher in psoriatic scales than in normal epidermis (3.2+/-2.3 and < 0.2 ng per mg, respectively), whereas hepoxilin A3 was not detected in any sample. Trioxilins were semiquantitated and referred to 12-hydroxyeicosatetraenoic acid, ratios of trioxilins A3 and B3 12-hydroxyeicosatetraenoic acid in psoriatic lesions were 0.65+/-0.23 and 0.32+/-0.28, respectively, and they were not detected in normal epidermis. The presence of a great amount of trioxilin A3 strongly suggests that hepoxilin A3 was present in psoriatic lesions and it was totally degraded to trioxilin A3 during the analysis procedure. Our results demonstrate that hepoxilins and trioxilins are produced by human skin in vivo and that the levels of these compounds are increased in psoriasis. The reported biologic activities of hepoxilins indicate that they could amplify and maintain the inflammatory response. Our results reinforce the idea that these compounds could play a role as mediators in the inflammatory response in skin, particularly in psoriasis.
我们最近发现，正常人类表皮在体外能产生相对大量的肝氧素和三氧素。因此，本研究的目的是证实这些化合物在银屑病皮损中的存在。采用高效液相色谱和气相色谱 - 质谱联用技术，对慢性稳定斑块状银屑病患者鳞屑提取物进行分析。我们发现，银屑病鳞屑中肝氧素B3的水平比正常表皮高16倍以上（分别为每毫克3.2±2.3纳克和<0.2纳克），而在任何样本中均未检测到肝氧素A3。对三氧素进行半定量分析，并以12 - 羟基二十碳四烯酸为参照，银屑病皮损中三氧素A3与12 - 羟基二十碳四烯酸的比值为0.65±0.23，三氧素B3与12 - 羟基二十碳四烯酸的比值为0.32±0.28，正常表皮中未检测到。大量三氧素A3的存在强烈表明，肝氧素A3存在于银屑病皮损中，且在分析过程中完全降解为三氧素A3。我们的结果表明，肝氧素和三氧素是人体皮肤在体内产生的，且这些化合物的水平在银屑病中升高。报道的肝氧素生物学活性表明，它们可放大并维持炎症反应。我们的结果强化了这样一种观点，即这些化合物可能在皮肤炎症反应中，特别是在银屑病中作为介质发挥作用。
