Taizo I, Sinichi A, Kawamura K
Taiyo Toyo Sanso Co. Ltd., Saitama, Japan.
PDA J Pharm Sci Technol. 1998 Jan-Feb;52(1):13-8.
A new type of concentration sensor for hydrogen peroxide vapor has been developed by making use of a semiconductor. Output from the vapor sensor has been shown to have a good linear relationship with the logarithm of the concentration of hydrogen peroxide vapor. Concentration of hydrogen peroxide vapor introduced into the sterilization chamber could be kept constant by monitoring the concentration of the hydrogen peroxide vapor continuously and controlling the vapor supply. Temperature and humidity have also been kept constant. D-values for B. stearothermophilus ATCC 12980 at various concentrations of hydrogen peroxide vapor have been determined by using the combination system of the hydrogen peroxide vapor sensor, the hydrogen peroxide vapor supplier, thermosensor and humidity sensor. D-values at the temperature of 30 degrees C and the absolute humidity of 0.7 mg H2O/L thus obtained, were 0.2 minutes at hydrogen peroxide concentration of 600 ppm and 1.2 minutes at 200 ppm at the temperature of 30 degrees C and 0.7 mg/L absolute humidity. D-values for B. stearothermophilus ATCC 12980 at various temperatures, humidity and levels of hydrogen peroxide concentration have also been determined. These fundamental data indicate that the sterilization by hydrogen peroxide vapor can be validated as precisely as steam sterilization by measuring and controlling the concentration of hydrogen peroxide vapor using a combination of the hydrogen peroxide concentration sensor and the vapor generator. Influence of temperature and humidity have also been studied. The hydrogen peroxide sensor has been calibrated and standardized by using the standard hydrogen peroxide vapor whose concentration has been determined by calculating partial pressure of hydrogen peroxide over the water-hydrogen peroxide solution.
利用半导体开发了一种新型的过氧化氢蒸汽浓度传感器。已证明该蒸汽传感器的输出与过氧化氢蒸汽浓度的对数具有良好的线性关系。通过连续监测过氧化氢蒸汽的浓度并控制蒸汽供应，可以使引入灭菌室的过氧化氢蒸汽浓度保持恒定。温度和湿度也保持恒定。通过使用过氧化氢蒸汽传感器、过氧化氢蒸汽供应器、温度传感器和湿度传感器的组合系统，测定了嗜热脂肪芽孢杆菌ATCC 12980在不同浓度过氧化氢蒸汽下的D值。在30℃温度和0.7mg H2O/L绝对湿度下，过氧化氢浓度为600ppm时，D值为0.2分钟；在30℃温度和0.7mg/L绝对湿度下，过氧化氢浓度为200ppm时，D值为1.2分钟。还测定了嗜热脂肪芽孢杆菌ATCC 12980在不同温度、湿度和过氧化氢浓度水平下的D值。这些基础数据表明，通过使用过氧化氢浓度传感器和蒸汽发生器的组合来测量和控制过氧化氢蒸汽的浓度，可以像蒸汽灭菌一样精确地验证过氧化氢蒸汽灭菌效果。还研究了温度和湿度的影响。通过使用由水 - 过氧化氢溶液上过氧化氢的分压计算确定浓度的标准过氧化氢蒸汽，对过氧化氢传感器进行了校准和标准化。
