Kimura Y, Nakano Y, Fujita K, Miyabe S, Imasaka S, Ishikawa Y, Sato M
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
Yeast. 1998 Feb;14(3):233-8. doi: 10.1002/(SICI)1097-0061(199802)14:3<233::AID-YEA216>3.0.CO;2-1.
Seven oligotrophic yeasts, which can grow in a 10(4)-fold dilution of malt-yeast-glucose-peptone medium (10(-4) YM), were mainly isolated from soil. These yeasts belong to the Cryptococcaceae. When inoculated at about 10(2) cells/ml in 10(-4) YM, the isolates grew to 1.4 x 10(3)-2.4 x 10(5) cells/ml after 3 days. Some culture collection yeasts fell into three groups according to their growth characteristics in 10(-4) YM, one group showing characteristics of the oligotrophic yeasts. The half-saturation values of uptake by the five isolated oligotrophic yeasts for D-glucose, L-leucine and L-amino acids were 6.0-25.0, 1.7-43.3 and 3.5-21.6 microM, respectively. The oligotrophic yeasts suspended in 10 mM-phosphate buffer (pH 6.0) had high tolerances for starvation, and remained more than 15% viable after 90 days of starvation.
七种贫营养酵母主要从土壤中分离得到，它们能够在麦芽酵母葡萄糖蛋白胨培养基（10⁻⁴YM）的10⁴倍稀释液中生长。这些酵母属于隐球酵母科。当以约10²个细胞/毫升的接种量接种于10⁻⁴YM中时，分离株在3天后生长至1.4×10³ - 2.4×10⁵个细胞/毫升。一些保藏中心的酵母根据它们在10⁻⁴YM中的生长特性分为三组，其中一组表现出贫营养酵母的特征。五种分离得到的贫营养酵母对D - 葡萄糖、L - 亮氨酸和L - 氨基酸的摄取半饱和值分别为6.0 - 25.0、1.7 - 43.3和3.5 - 21.6微摩尔。悬浮于10毫摩尔磷酸盐缓冲液（pH 6.0）中的贫营养酵母对饥饿具有高耐受性，在饥饿90天后仍有超过15%的存活率。
