Janković B D, Isaković K, Marković B M, Rajcević M, Knezević Z
Exp Hematol. 1976 Jul;4(4):246-55.
Chickens sham-bursectomized as 52- to 64-hours-old embryos (SBx), chickens bursectomized at hatching (NeoBx) and chickens from which the bursal primordium was removed at 52-64 h of embryonation (EBx) were immunized with guinea pig red blood cells when 21 days old. Following the second injection of antigen, EBx chickens were found to elaborate ME-sensitive and ME-resistant hemagglutinins, to produce direct and indirect plaque-forming cells, and to contain lymphocytes bearing a specific bursa (Bu) antigen. These immune properties were more often expressed in sham-bursectomized and neonatally bursectomized chickens. Cytomorphological changes in the spleen, bone marrow and cecal tonsil of EBx chickens were typical for birds lacking the bursa. The number of lymphocytes and plasma cells in the bone marrow and liver of SBx, NeoBx and EBx chickens was very low. These results suggest that a very early embryonic bursectomy of chickens does not abolish humoral immunity completely and does not prevent the differentiation of Bu antigen-bearing lymphocytes.
将52至64小时龄胚胎进行假法氏囊切除的鸡（SBx）、孵化时进行法氏囊切除的鸡（NeoBx）以及在胚胎发育52至64小时时切除法氏囊原基的鸡（EBx）在21日龄时用豚鼠红细胞进行免疫。在第二次注射抗原后，发现EBx鸡能产生对甲基乙二醛敏感和耐药的血凝素，产生直接和间接的空斑形成细胞，并含有带有特定法氏囊（Bu）抗原的淋巴细胞。这些免疫特性在假法氏囊切除和新生期法氏囊切除的鸡中更常表现出来。EBx鸡的脾脏、骨髓和盲肠扁桃体的细胞形态学变化是缺乏法氏囊的鸟类的典型特征。SBx、NeoBx和EBx鸡的骨髓和肝脏中的淋巴细胞和浆细胞数量非常低。这些结果表明，鸡的极早期胚胎法氏囊切除并不会完全消除体液免疫，也不会阻止带有Bu抗原的淋巴细胞的分化。
