Baer G M
Dev Biol Stand. 1976;33:417-23.
Foxes wer immunized orally with an attenuated rabies vaccine, ERA, grown on BHK cells. The liquid vaccine was placed in plastic straws, which in turn were incorporated into smoked sausage baits, acceptable to and readily ingested by the animals. When the baits were bitten and the meat swallowed, an oral immunizing dose of vaccine resulted in circulating antibody titers in foxes (and dogs); the animals with antibody resisted a "street" rabies virus challenge that killed unvaccinated controls. The immunization was strictly lingual and buccal, and foxes with interrupted esophagi developed antibody only if the vaccine was deposited in the mouth, while those given a similar dose in the ventral esophagostomy opening (below the interruption and close to the stomach) failed to develop antibody. A casein hydrolysate derivative resulted in such stabilization of the liquid that even when baits were held at 35 degree C for 3 days, similar to extreme field conditions, an immunizing titer for foxes (greater than or equal to 10(4.5)LD50) was still maintained.
用在BHK细胞上培养的减毒狂犬病疫苗ERA对狐狸进行口服免疫。液体疫苗被放置在塑料吸管中，然后将吸管放入烟熏香肠诱饵中，这些诱饵动物可以接受并容易摄取。当诱饵被咬且肉被吞下时，口服免疫剂量的疫苗会使狐狸（和狗）体内产生循环抗体滴度；有抗体的动物能抵抗“街”狂犬病病毒攻击，而未接种疫苗的对照动物则会死亡。免疫严格局限于舌部和颊部，食管中断的狐狸只有在疫苗被置于口腔时才会产生抗体，而在腹侧食管造口开口处（在中断下方且靠近胃部）给予相似剂量疫苗的狐狸则未能产生抗体。酪蛋白水解物衍生物使液体如此稳定，以至于即使诱饵在35摄氏度下放置3天，类似于极端野外条件，对狐狸的免疫滴度（大于或等于10(4.5)LD50）仍能维持。
