Piechota H J, Dahms S E, Nunes L S, Dahiya R, Lue T F, Tanagho E A
Department of Urology, University of California School of Medicine, San Francisco, USA.
J Urol. 1998 May;159(5):1717-24. doi: 10.1097/00005392-199805000-00100.
To assess the response of rat urinary bladder regenerated by the homologous bladder acellular matrix graft (BAMG) to in vitro electrical and pharmacologic stimuli.
In Sprague-Dawley rats, partial cystectomy (>50%) was performed, followed by BAMG augmentation cystoplasty. After 4 months, organ bath studies of tissue strips in 10 were used to compare the contractility of the BAMG regenerates and the corresponding host detrusor smooth muscle.
The BAMG regenerates exhibited contractile activity to electrical field stimulation and a qualitatively identical pattern of response to muscarinic, purinergic, alpha- and beta-adrenergic drug administration and nitric oxide. At 4 months after surgery, the maximum forces of contraction of the BAMG regenerates to carbachol stimulation amounted to close to 80% of the host bladder response. With electrical field stimulation, they equaled 44% and 62% of the host bladder response after 2.5 and 4 months, respectively. Histological and immunohistochemical studies confirmed the presence of receptors for neurotransmitters that these functional in vitro studies implied.
The present study provides further evidence that augmentation cystoplasty with the BAMG leads to functional regeneration of the rat bladder detrusor smooth muscle.
评估同种异体膀胱脱细胞基质移植物（BAMG）再生的大鼠膀胱对体外电刺激和药物刺激的反应。
对Sprague-Dawley大鼠进行部分膀胱切除术（>50%），随后行BAMG扩大膀胱成形术。4个月后，对10只大鼠的组织条进行器官浴研究，以比较BAMG再生组织与相应宿主逼尿肌平滑肌的收缩性。
BAMG再生组织对电场刺激表现出收缩活性，对毒蕈碱、嘌呤能、α和β肾上腺素能药物及一氧化氮的反应模式在性质上相同。术后4个月，BAMG再生组织对卡巴胆碱刺激的最大收缩力接近宿主膀胱反应的80%。在电场刺激下，术后2.5个月和4个月时，它们分别相当于宿主膀胱反应的44%和62%。组织学和免疫组化研究证实了这些体外功能研究中所暗示的神经递质受体的存在。
本研究进一步证明，用BAMG进行扩大膀胱成形术可导致大鼠膀胱逼尿肌平滑肌的功能再生。
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