抗原性糖脂酶联免疫吸附测定在肺结核早期诊断中的应用。

Application of ELISA with antigenic glycolipids in early diagnosis of pulmonary tuberculosis.

作者信息

Brânaru-Gheorghiu M, Răileanu M, Ciomu E, Niculescu D, Popa M, Stavri H, Stavri D

机构信息

Pneumophthisiology and Pneumology Unit, Bucharest, Romania.

出版信息

Roum Arch Microbiol Immunol. 1997 Jan-Jun;56(1-2):55-61.

PMID:9558976

Abstract

Antibodies against M. tuberculosis antigenic glycolipids were determined by enzyme-linked immunosorbent assay (ELISA) in 80 sera from patients suspected of pulmonary tuberculosis, with suggestive clinical signs and radiological abnormalities, but smears negative. The test was also performed on 68 control sera from patients with bacteriologically confirmed pulmonary tuberculosis, in different stages of disease. We obtained the test sensitivity of 89.5% for the confirmed tuberculosis patient sera. Low levels of specific antibodies and the sensitivity of 75% were found when using ELISA in smears negative patients at the onset of clinical tuberculosis. The experimental results showed that ELISA could be used as a supporting test in early diagnosis of pulmonary tuberculosis.

摘要

采用酶联免疫吸附测定法（ELISA），对80份疑似肺结核患者的血清进行结核分枝杆菌抗原糖脂抗体检测。这些患者有提示性的临床症状和放射学异常，但涂片检查为阴性。同时，对68份来自处于疾病不同阶段、经细菌学确诊为肺结核患者的对照血清也进行了该检测。对于确诊的肺结核患者血清，我们获得的检测灵敏度为89.5%。在临床肺结核发病初期涂片阴性的患者中使用ELISA检测时，发现特异性抗体水平较低，灵敏度为75%。实验结果表明，ELISA可作为肺结核早期诊断的辅助检测方法。