大肠杆菌rho因子可诱导酵母RNA聚合酶II释放，但不会诱导聚合酶I或III释放。
Escherichia coli rho factor induces release of yeast RNA polymerase II but not polymerase I or III.
作者信息
Lang W H, Platt T, Reeder R H
机构信息
Basic Sciences Division, Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4900-5. doi: 10.1073/pnas.95.9.4900.
Abstract
Purified RNA polymerase II (pol II) from the yeast Saccharomyces cerevisiae pauses without releasing at many locations during in vitro transcription. Pausing can be induced by intrinsic DNA sequence as well as by specific DNA bound proteins such as the RNA pol I termination factor, Reb1p, or lac repressor. Addition of rho termination factor from E. coli induces RNA pol II to release at all of these pause sites. Rho-induced release of pol II requires both a rho binding site in the transcript upstream of the pause sites as well as hydrolysis of ATP. In contrast, rho factor has no effect on either pausing or release by RNA pol I or III. When combined with previous observations, these results suggest that RNA pol II may terminate by a mechanism closely related to the rho-dependent mechanism of prokaryotes. In contrast, pol I and III appear to utilize a mechanism more related to the rho-independent terminators of prokaryotes.
摘要
从酿酒酵母中纯化得到的RNA聚合酶II（pol II）在体外转录过程中会在许多位置暂停，但不会释放。暂停可由内在DNA序列以及特定的DNA结合蛋白诱导，如RNA聚合酶I终止因子Reb1p或乳糖阻遏物。添加来自大肠杆菌的rho终止因子会诱导RNA聚合酶II在所有这些暂停位点释放。rho诱导的pol II释放既需要在暂停位点上游的转录本中有rho结合位点，也需要ATP水解。相比之下，rho因子对RNA聚合酶I或III的暂停或释放均无影响。结合先前的观察结果，这些结果表明RNA聚合酶II可能通过一种与原核生物rho依赖性机制密切相关的机制终止转录。相比之下，聚合酶I和III似乎利用了一种与原核生物rho非依赖性终止子更相关的机制。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8433-8. doi: 10.1073/pnas.94.16.8433.
2
Transcriptional termination signals for RNA polymerase II in fission yeast.裂殖酵母中RNA聚合酶II的转录终止信号
EMBO J. 1997 Jun 16;16(12):3633-43. doi: 10.1093/emboj/16.12.3633.
3
Multiple interactions stabilize a single paused transcription intermediate in which hairpin to 3' end spacing distinguishes pause and termination pathways.多种相互作用稳定了单个暂停转录中间体，其中发夹结构与3'端的间距决定了暂停和终止途径。
J Mol Biol. 1997 Apr 25;268(1):54-68. doi: 10.1006/jmbi.1997.0935.
4
Rho-dependent termination of transcription is governed primarily by the upstream Rho utilization (rut) sequences of a terminator.转录的Rho依赖性终止主要由终止子的上游Rho利用（rut）序列控制。
J Biol Chem. 1996 Aug 30;271(35):21597-603. doi: 10.1074/jbc.271.35.21597.
5
Transcription processivity: protein-DNA interactions holding together the elongation complex.转录持续性：使延伸复合物保持在一起的蛋白质 - DNA 相互作用。
Science. 1996 Jul 12;273(5272):211-7. doi: 10.1126/science.273.5272.211.
6
Poly(A) signals and transcriptional pause sites combine to prevent interference between RNA polymerase II promoters.聚腺苷酸（Poly(A)）信号与转录暂停位点共同作用，以防止RNA聚合酶II启动子之间的干扰。
EMBO J. 1993 Jun;12(6):2539-48. doi: 10.1002/j.1460-2075.1993.tb05909.x.
7
Transcription termination.转录终止。
Crit Rev Biochem Mol Biol. 1993;28(1):1-30. doi: 10.3109/10409239309082571.
8
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Mol Cell Biol. 1993 Jan;13(1):649-58. doi: 10.1128/mcb.13.1.649-658.1993.
9
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10
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J Mol Biol. 1994 Jan 28;235(4):1173-92. doi: 10.1006/jmbi.1994.1072.
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