钠通道选择性。对内部通透离子浓度的依赖性。
Sodium channel selectivity. Dependence on internal permeant ion concentration.
作者信息
Cahalan M, Begenisich T
出版信息
J Gen Physiol. 1976 Aug;68(2):111-25. doi: 10.1085/jgp.68.2.111.
Abstract
The selectivity of sodium channels in squid axon membranes was investigated with widely varying concentrations of internal ions. The selectivity ratio, PNa/PK, determined from reversal potentials decreases from 12.8 to 5.7 to 3.5 as the concentration of internal potassium is reduced from 530 to 180 to 50 mM, respectively. The internal KF perfusion medium can be diluted by tetramethylammonium (TMA), Tris, or sucrose solutions with the same decrease in PNa/PK. The changes in the selectivity ratio depend upon internal permeant ion concentration rather than ionic strength, membrane potential, or chloride permeability. Lowering the internal concentration of cesium, rubidium, guanidnium, or ammonium also reduces PNa/Pion. The selective sequence of the sodium channel is: Na greater than guanidinium greater than ammonium greater than K greater than Rb greater than Cs.
摘要
利用浓度差异极大的内部离子，对枪乌贼轴突膜中钠通道的选择性进行了研究。由反转电位确定的选择性比率PNa/PK，随着内部钾浓度分别从530 mM降至180 mM再降至50 mM，从12.8降至5.7再降至3.5。内部KF灌注介质可用四甲基铵（TMA）、Tris或蔗糖溶液稀释，PNa/PK也会有相同程度的降低。选择性比率的变化取决于内部通透离子浓度，而非离子强度、膜电位或氯离子通透性。降低铯、铷、胍或铵的内部浓度也会降低PNa/P离子。钠通道的选择性顺序为：Na＞胍＞铵＞K＞Rb＞Cs。
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