当翻译起始受到限制时，Upf1和Upf2蛋白介导正常的酵母mRNA降解。
Upf1 and Upf2 proteins mediate normal yeast mRNA degradation when translation initiation is limited.
作者信息
Barnes C A
机构信息
Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada.
出版信息
Nucleic Acids Res. 1998 May 15;26(10):2433-41. doi: 10.1093/nar/26.10.2433.
Abstract
mRNA degradation is coupled with the process of mRNA translation. For example, an mRNA molecule, on which translation is prematurely terminated because of a nonsense codon, may be rapidly degraded. This nonsense-mediated mRNA decay in the yeast Saccharomyces cerevisiae is mediated by the Upf1 and Upf2 proteins. Yeast mRNAs can also be selectively destabilized by limiting the rate of translation initiation. Two such destabilized mRNAs, from the SSA1 and SSA2 genes, have been identified using temperature-sensitive mutations affecting the Prt1 component of eukaryotic initiation factor 3. For SSA1 and SSA2 mRNAs, and for structurally modified SSA mRNA derivatives, I show here that degradation is triggered when translation initiation is limited but ongoing. This initiation-dependent mRNA degradation is limited to a subset of mRNAs that includes at least those from the SSA1 and SSA2 genes, and occurs through Upf1- and Upf2-mediated processes, although sequence elements characteristic of nonsense-mediated decay are not evident in these mRNAs.
摘要
mRNA降解与mRNA翻译过程相关联。例如，一个mRNA分子，由于无义密码子导致翻译提前终止，可能会迅速降解。酿酒酵母中的这种无义介导的mRNA降解是由Upf1和Upf2蛋白介导的。酵母mRNA也可以通过限制翻译起始速率而被选择性地不稳定化。利用影响真核起始因子3的Prt1组分的温度敏感突变，已经鉴定出两个这样不稳定的mRNA，分别来自SSA1和SSA2基因。对于SSA1和SSA2 mRNA，以及结构修饰的SSA mRNA衍生物，我在此表明，当翻译起始受到限制但仍在进行时，降解就会被触发。这种起始依赖性mRNA降解仅限于一个mRNA子集，其中至少包括来自SSA1和SSA2基因的那些mRNA，并且通过Upf1和Upf2介导的过程发生，尽管在这些mRNA中无义介导的降解的特征性序列元件并不明显。
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