Remarque E J, de Bruijn I A, Boersma W J, Masurel N, Ligthart G J
Department of Internal Medicine, Leiden University Medical Centre, The Netherlands.
J Med Virol. 1998 May;55(1):82-7. doi: 10.1002/(sici)1096-9071(199805)55:1<82::aid-jmv13>3.0.co;2-1.
To determine the influence of ageing per se as well as of priming histories on the antibody response to influenza vaccination, haemagglutination inhibition (HI), ELISA IgG, IgA, IgM and neutralizing antibody titres were studied in 43 healthy young subjects (mean age 23 years) and 55 healthy elderly people (mean age 79 years). The HI and ELISA lgG responses to the A/Guizhou/54/89 strain (H3N2) for which both the young and the elderly had similar priming histories were equal. By contrast, the HI and IgG responses to A/Taiwan/1/86 (H1N1), where the priming histories were different, were lower in the elderly (P < 0.05). Influenza-specific IgA responses in the elderly tended to be higher for all vaccine strains. Influenza-specific postvaccination IgM titres were similar or tended to be higher in the elderly. A subgroup of elderly subjects (18%) who did not express HI activity to the A/Taiwan/1/86 (H1N1) vaccine strain, reacted in the HI assay with the closely related A/Singapore/6/86 (H1N1) strain. These elderly people, however, produced lgG antibodies which neutralized A/Taiwan/1/86 virus in vitro. It is concluded that the elderly are capable of mounting antibody responses similar to those observed in the young. Moreover, the observed age-related differences in antibody responses to H1N1 strains are probably not due to ageing of the immune system itself, but are determined by differences in priming histories.
为了确定衰老本身以及免疫初免史对流感疫苗抗体反应的影响，我们对43名健康年轻受试者（平均年龄23岁）和55名健康老年人（平均年龄79岁）的血凝抑制（HI）、ELISA IgG、IgA、IgM和中和抗体滴度进行了研究。年轻和老年受试者对A/贵州/54/89株（H3N2）的HI和ELISA IgG反应相同，因为他们的免疫初免史相似。相比之下，对A/台湾/1/86（H1N1）株的HI和IgG反应，由于免疫初免史不同，老年人的反应较低（P<0.05）。老年人对所有疫苗株的流感特异性IgA反应往往更高。接种疫苗后，老年人的流感特异性IgM滴度相似或往往更高。一组对A/台湾/1/86（H1N1）疫苗株无HI活性的老年受试者（18%），在HI试验中对密切相关的A/新加坡/6/86（H1N1）株有反应。然而，这些老年人产生的IgG抗体在体外可中和A/台湾/1/86病毒。结论是，老年人能够产生与年轻人相似的抗体反应。此外，观察到的老年人对H1N1株抗体反应的年龄差异可能不是由于免疫系统本身的衰老，而是由免疫初免史的差异决定的。
