Matee M I, Mikx F H, Maselle S Y, Van Palenstein Helderman W H
Department of Microbiology and Immunology, Muhimbili University College of Health Sciences, Dar-es-Salaam, Tanzania.
Afr Dent J. 1993;7:1-5.
The establishment of S. mutans together with A. viscosus was investigated in dental plaque of specific pathogen-free (SPF) rats fed different carbohydrate diets. Two Tanzanian S.mutans strains MM3 and MM24 and one Tanzanian A. viscosus strain MM13 were used for this purpose. The basic diet consisting of 32% skim-milk, 7% yeast extract and 1% soy bean oil was supplemented with either 10% lactose and 50% corn flour, or 10% glucose and 50% corn flour, or 60% amylum or 60% wheat flour. S. mutans and A. viscosus were enumerated twenty days after inoculation. S. mutans counts were high irrespective of the dietary regime. A. viscosus counts in the glucose, lactose and amylum groups were of the same magnitude and significantly higher than those of the wheat flour group. The hypothesis that the establishment of S. mutans in sucrose free diets could be facilitated by the extracellular polysaccharides produced by A. viscosus was not supported by the present data. The finding that S. mutans can establish in high number in dental plaque of SPF rats in the absence of sucrose corroborates previous reports indicating high S. mutans counts in African populations with a low sucrose intake.
在喂食不同碳水化合物饮食的无特定病原体（SPF）大鼠的牙菌斑中，研究了变形链球菌与粘性放线菌的定植情况。为此使用了两株坦桑尼亚变形链球菌菌株MM3和MM24以及一株坦桑尼亚粘性放线菌菌株MM13。由32%脱脂牛奶、7%酵母提取物和1%大豆油组成的基础饮食分别补充10%乳糖和50%玉米粉、或10%葡萄糖和50%玉米粉、或60%淀粉或60%小麦粉。接种二十天后对变形链球菌和粘性放线菌进行计数。无论饮食方案如何，变形链球菌的计数都很高。葡萄糖、乳糖和淀粉组中粘性放线菌的计数处于相同水平，且显著高于小麦粉组。粘性放线菌产生的细胞外多糖可促进无蔗糖饮食中变形链球菌的定植这一假设未得到当前数据的支持。变形链球菌在无蔗糖的情况下能在SPF大鼠牙菌斑中大量定植这一发现证实了先前的报告，即蔗糖摄入量低的非洲人群中变形链球菌计数很高。
