Jaye A, Magnusen A F, Whittle H C
Medical Research Council Laboratories, Banjul, The Gambia, West Africa.
J Infect Dis. 1998 May;177(5):1282-9. doi: 10.1086/515271.
The study of cytotoxic T cell (CTL) responses to measles polypeptides in persons with different HLA frequencies will provide information for the design of new vaccines. Peripheral blood mononuclear cells derived from African blacks and Caucasians were stimulated with measles virus-infected autologous cells and tested in a standard 51Cr-release assay against autologous B cells infected with vaccinia virus recombinants expressing measles virus antigens. The proportion of subjects who generated CTL to the fusion, hemagglutinin, and nucleoprotein antigens was 43%, 38%, and 28%, respectively. The use of HLA-mismatched targets showed killing to be restricted by both HLA class I and class II antigens, although CD8-mediated class I cytotoxicity predominated. Measles vaccine boosted CTL responses in subjects with low initial activity. These data suggest that the fusion and hemagglutinin proteins are important targets for the measles CTL response.
对具有不同HLA频率的人群中细胞毒性T细胞（CTL）对麻疹多肽反应的研究将为新型疫苗的设计提供信息。从非洲黑人和白种人获取的外周血单个核细胞用麻疹病毒感染的自体细胞进行刺激，并在标准的51Cr释放试验中针对用表达麻疹病毒抗原的痘苗病毒重组体感染的自体B细胞进行检测。产生针对融合抗原、血凝素抗原和核蛋白抗原的CTL的受试者比例分别为43%、38%和28%。使用HLA不匹配的靶细胞显示杀伤作用受HLA I类和II类抗原限制，尽管CD8介导的I类细胞毒性占主导。麻疹疫苗增强了初始活性较低的受试者的CTL反应。这些数据表明融合蛋白和血凝素蛋白是麻疹CTL反应的重要靶点。
