• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Properties of mutant SHV-5 beta-lactamases constructed by substitution of isoleucine or valine for methionine at position 69.通过将69位的甲硫氨酸替换为异亮氨酸或缬氨酸构建的突变型SHV-5β-内酰胺酶的特性
Antimicrob Agents Chemother. 1998 May;42(5):1281-3. doi: 10.1128/AAC.42.5.1281.
2
Aspartic acid for asparagine substitution at position 276 reduces susceptibility to mechanism-based inhibitors in SHV-1 and SHV-5 beta-lactamases.天冬氨酸替代276位的天冬酰胺会降低SHV-1和SHV-5β-内酰胺酶对基于机制的抑制剂的敏感性。
J Antimicrob Chemother. 1999 Jan;43(1):23-9. doi: 10.1093/jac/43.1.23.
3
Selection and characterization of beta-lactam-beta-lactamase inactivator-resistant mutants following PCR mutagenesis of the TEM-1 beta-lactamase gene.TEM-1β-内酰胺酶基因经PCR诱变后β-内酰胺-β-内酰胺酶灭活剂抗性突变体的筛选与鉴定
Antimicrob Agents Chemother. 1998 Jul;42(7):1542-8. doi: 10.1128/AAC.42.7.1542.
4
Substitutions at position 105 in SHV family β-lactamases decrease catalytic efficiency and cause inhibitor resistance.在 SHV 家族β-内酰胺酶的位置 105 发生取代会降低催化效率并导致抑制剂耐药性。
Antimicrob Agents Chemother. 2012 Nov;56(11):5678-86. doi: 10.1128/AAC.00711-12. Epub 2012 Aug 20.
5
Amino acid substitutions causing inhibitor resistance in TEM beta-lactamases compromise the extended-spectrum phenotype in SHV extended-spectrum beta-lactamases.导致TEMβ-内酰胺酶产生抑制剂耐药性的氨基酸取代会损害SHV超广谱β-内酰胺酶的超广谱表型。
J Antimicrob Chemother. 2001 May;47(5):547-54. doi: 10.1093/jac/47.5.547.
6
Kinetic analysis of an inhibitor-resistant variant of the OHIO-1 beta-lactamase, an SHV-family class A enzyme.SHV家族A类酶OHIO-1β-内酰胺酶的一种抑制剂抗性变体的动力学分析。
Biochem J. 1998 Jul 15;333 ( Pt 2)(Pt 2):395-400. doi: 10.1042/bj3330395.
7
Detrimental effect of the combination of R164S with G238S in TEM-1 beta-lactamase on the extended-spectrum activity conferred by each single mutation.TEM-1β-内酰胺酶中R164S与G238S组合对每个单突变赋予的超广谱活性的有害影响。
J Antimicrob Chemother. 2000 Jan;45(1):101-4. doi: 10.1093/jac/45.1.101.
8
Substitution of Thr for Ala-237 in TEM-17, TEM-12 and TEM-26: alterations in beta-lactam resistance conferred on Escherichia coli.TEM-17、TEM-12和TEM-26中丙氨酸-237被苏氨酸取代：对大肠杆菌β-内酰胺抗性的影响
FEMS Microbiol Lett. 2001 Jul 10;201(1):37-40. doi: 10.1111/j.1574-6968.2001.tb10729.x.
9
New system based on site-directed mutagenesis for highly accurate comparison of resistance levels conferred by SHV beta-lactamases.基于定点诱变的新系统，用于高度准确地比较SHVβ-内酰胺酶赋予的耐药水平。
Antimicrob Agents Chemother. 1995 Aug;39(8):1726-30. doi: 10.1128/AAC.39.8.1726.
10
Substitution of Arg-244 by Cys or Ser in SHV-1 and SHV-5 beta-lactamases confers resistance to mechanism-based inhibitors and reduces catalytic efficiency of the enzymes.在SHV-1和SHV-5β-内酰胺酶中，将第244位的精氨酸替换为半胱氨酸或丝氨酸会赋予对基于机制的抑制剂的抗性，并降低酶的催化效率。
FEMS Microbiol Lett. 1998 Mar 1;160(1):49-54. doi: 10.1111/j.1574-6968.1998.tb12889.x.

引用本文的文献

1
Copy number flexibility facilitates heteroresistance to increasing antibiotic pressure and threatens the beta-lactam pipeline.拷贝数灵活性促进了对不断增加的抗生素压力的异质性耐药，并威胁到β-内酰胺类药物研发进程。
Nat Commun. 2025 Jul 1;16(1):5721. doi: 10.1038/s41467-025-60828-9.
2
Diversity, functional classification and genotyping of SHV β-lactamases in .在中国临床分离的大肠埃希菌和肺炎克雷伯菌中 SHV 型β-内酰胺酶的多样性、功能分类和基因分型。
Microb Genom. 2024 Oct;10(10). doi: 10.1099/mgen.0.001294.
3
Non-catalytic-Region Mutations Conferring Transition of Class A β-Lactamases Into ESBLs.赋予A类β-内酰胺酶向超广谱β-内酰胺酶转变的非催化区域突变
Front Mol Biosci. 2020 Nov 27;7:598998. doi: 10.3389/fmolb.2020.598998. eCollection 2020.
4
Construction and characterization of mutants of the TEM-1 beta-lactamase containing amino acid substitutions associated with both extended-spectrum resistance and resistance to beta-lactamase inhibitors.构建并鉴定含有与超广谱耐药性和对β-内酰胺酶抑制剂耐药性相关氨基酸取代的TEM-1β-内酰胺酶突变体。
Antimicrob Agents Chemother. 1999 Aug;43(8):1881-7. doi: 10.1128/AAC.43.8.1881.

本文引用的文献

1
Construction and characterization of an OHIO-1 beta-lactamase bearing Met69Ile and Gly238Ser mutations.携带Met69Ile和Gly238Ser突变的OHIO-1β-内酰胺酶的构建与表征
Antimicrob Agents Chemother. 1997 Sep;41(9):1940-3. doi: 10.1128/AAC.41.9.1940.
2
A complex mutant of TEM-1 beta-lactamase with mutations encountered in both IRT-4 and extended-spectrum TEM-15, produced by an Escherichia coli clinical isolate.一种由大肠杆菌临床分离株产生的TEM-1β-内酰胺酶复合突变体，具有在IRT-4和超广谱TEM-15中均出现的突变。
Antimicrob Agents Chemother. 1997 Jun;41(6):1322-5. doi: 10.1128/AAC.41.6.1322.
3
Emergence of an inhibitor-resistant beta-lactamase (SHV-10) derived from an SHV-5 variant.源自SHV-5变体的耐抑制剂β-内酰胺酶（SHV-10）的出现。
Antimicrob Agents Chemother. 1997 Apr;41(4):838-40. doi: 10.1128/AAC.41.4.838.
4
Tazobactam is a potent inactivator of selected inhibitor-resistant class A beta-lactamases.他唑巴坦是一种对特定的耐抑制剂A类β-内酰胺酶有效的抑制剂。
FEMS Microbiol Lett. 1997 Mar 1;148(1):59-62. doi: 10.1111/j.1574-6968.1997.tb10267.x.
5
Comparative evaluation of the inhibitory activities of the novel penicillanic acid sulfone Ro 48-1220 against beta-lactamases that belong to groups 1, 2b, and 2be.新型青霉烷酸砜Ro 48 - 1220对1、2b和2be组β-内酰胺酶抑制活性的比较评价
Antimicrob Agents Chemother. 1997 Feb;41(2):475-7. doi: 10.1128/AAC.41.2.475.
6
Evolution and dissemination of beta-lactamases accelerated by generations of beta-lactam antibiotics.几代β-内酰胺类抗生素加速了β-内酰胺酶的进化与传播。
Clin Infect Dis. 1997 Jan;24 Suppl 1:S19-45. doi: 10.1093/clinids/24.supplement_1.s19.
7
Extended-spectrum and inhibitor-resistant TEM-type beta-lactamases: mutations, specificity, and three-dimensional structure.超广谱和耐抑制剂的TEM型β-内酰胺酶：突变、特异性及三维结构
Antimicrob Agents Chemother. 1995 Dec;39(12):2593-601. doi: 10.1128/AAC.39.12.2593.
8
Role of Ser-238 and Lys-240 in the hydrolysis of third-generation cephalosporins by SHV-type beta-lactamases probed by site-directed mutagenesis and three-dimensional modeling.通过定点诱变和三维建模探究Ser-238和Lys-240在SHV型β-内酰胺酶水解第三代头孢菌素中的作用
J Biol Chem. 1993 Feb 15;268(5):3690-7.
9
Characterization of a new TEM-type beta-lactamase resistant to clavulanate, sulbactam, and tazobactam in a clinical isolate of Escherichia coli.一株临床分离的大肠杆菌中对克拉维酸、舒巴坦和他唑巴坦耐药的新型TEM型β-内酰胺酶的特性分析
Antimicrob Agents Chemother. 1993 Oct;37(10):2059-63. doi: 10.1128/AAC.37.10.2059.
10
Characterization of a beta-lactamase from Clostridium clostridioforme.来自艰难梭状芽孢杆菌的一种β-内酰胺酶的特性分析。
J Antimicrob Chemother. 1994 Jan;33(1):33-40. doi: 10.1093/jac/33.1.33.

通过将69位的甲硫氨酸替换为异亮氨酸或缬氨酸构建的突变型SHV-5β-内酰胺酶的特性

Properties of mutant SHV-5 beta-lactamases constructed by substitution of isoleucine or valine for methionine at position 69.

作者信息

Giakkoupi P, Miriagou V, Gazouli M, Tzelepi E, Legakis N J, Tzouvelekis L S

机构信息

Department of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.

出版信息

Antimicrob Agents Chemother. 1998 May;42(5):1281-3. doi: 10.1128/AAC.42.5.1281.

DOI:10.1128/AAC.42.5.1281
PMID:9593168
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC105805/
Abstract

The effect of replacement of Met-69 by Ile or Val on the properties of the extended-spectrum beta-lactamase SHV-5 was studied. Mutant enzymes were constructed by site-specific mutagenesis and expressed under isogenic conditions in Escherichia coli DH5alpha cells. Compared with SHV-5, the mutant beta-lactamases conferred lower levels of beta-lactam resistance and were less efficient in hydrolyzing ampicillin, cephalothin, and cefotaxime. The substitutions rendered SHV-5 less susceptible to inhibition by clavulanate, sulbactam, and tazobactam; however, the MICs of penicillin-inhibitor combinations remained similar, suggesting an attenuation of penicillinase activity.

摘要

研究了将甲硫氨酸-69替换为异亮氨酸或缬氨酸对超广谱β-内酰胺酶SHV-5性质的影响。通过定点诱变构建突变酶，并在同基因条件下在大肠杆菌DH5α细胞中表达。与SHV-5相比，突变型β-内酰胺酶赋予的β-内酰胺抗性水平较低，并且在水解氨苄西林、头孢噻吩和头孢噻肟方面效率较低。这些取代使SHV-5对克拉维酸、舒巴坦和他唑巴坦的抑制作用更不敏感；然而，青霉素抑制剂组合的最低抑菌浓度保持相似，表明青霉素酶活性减弱。